PSMD7 Mouse mAb: FAQs for Research Use

PSMD7 Mouse mAb is a monoclonal antibody raised in mouse, designed to specifically recognize the PSMD7 subunit of the 26S proteasome regulatory complex for research applications.

Product SKU

Ab350659

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

PSMD7 (26S Proteasome Regulatory Subunit 7)

Formulation

PBS (Phosphate-Buffered Saline) only

Host Species

Mouse

Clone Reference

KC-36422 (absea reference)

Overview of PSMD7 and Its Biological Role

PSMD7 (Proteasome 26S Subunit, Non-ATPase 7), also known as MOV34 or p40, is a non-ATPase regulatory subunit of the 19S regulatory particle of the 26S proteasome. The 26S proteasome is the principal ATP-dependent protease responsible for ubiquitin-mediated protein degradation in eukaryotic cells. PSMD7 contains a MPN (Mpr1p and Pad1p N-terminal) domain and is involved in the recognition and processing of ubiquitinated substrates. Research into PSMD7 has implications for understanding protein homeostasis, cell cycle regulation, and disease pathways including cancer and neurodegeneration.

Product Specifications and Formulation

The PSMD7 Mouse mAb (SKU: Ab350659) is supplied as a purified monoclonal antibody formulated in PBS (Phosphate-Buffered Saline) only, without additional stabilizers such as glycerol or BSA unless otherwise stated on the product datasheet. The antibody is generated in mouse and references the clone KC-36422. Researchers should consult the full product datasheet for precise concentration, total volume, and any lot-specific quality control data prior to use.

Note: Because the formulation contains PBS only, researchers planning long-term storage should consider adding a carrier protein (e.g., BSA to 0.1%) or a cryoprotectant (e.g., glycerol to 50%) and aliquoting to avoid repeated freeze-thaw cycles, unless specific datasheet guidance indicates otherwise.

Recommended Storage and Handling

For short-term storage (up to 1 month), the PSMD7 Mouse mAb may be kept at 4°C. For long-term storage, aliquot the antibody and store at -20°C or -80°C to preserve activity. Avoid repeated freeze-thaw cycles, as these can lead to protein aggregation and loss of binding activity. Since the supplied formulation is PBS only, it is advisable to add a stabilizing agent if long-term stability is required. Keep the antibody away from light and do not store in frost-free freezers that undergo temperature cycling.

Typical Research Applications

Monoclonal antibodies targeting PSMD7 are typically employed in a variety of immunological research techniques. Common applications include:

• Western Blotting (WB): Detection of PSMD7 protein expression in cell or tissue lysates.
• Immunoprecipitation (IP): Isolation of PSMD7-containing protein complexes for downstream analysis.
• Immunofluorescence (IF): Subcellular localization studies of PSMD7 in fixed cells.
• Immunohistochemistry (IHC): Detection of PSMD7 in formalin-fixed, paraffin-embedded tissue sections.
• Flow Cytometry (FC): Quantification of PSMD7 expression at the single-cell level.

Researchers should validate the antibody in their specific experimental system and consult the product datasheet for confirmed application data and recommended dilutions.

Ordering and Customer Support

The PSMD7 Mouse mAb is available from Aladdin Scientific under SKU Ab350659. To place an order, researchers and procurement personnel may use the Aladdin Scientific online catalog or contact the sales team directly. Bulk pricing, institutional purchase orders, and custom packaging inquiries are handled by the Aladdin Scientific customer service team. For technical questions regarding application, validation, or troubleshooting, please reach out to our technical support team with your lot number and experimental details.

Returns and refunds are subject to Aladdin Scientific's standard return policy. Please refer to the Returns and Refunds section of the website or contact customer service for guidance specific to antibody products.

Frequently asked questions

What is the formulation of PSMD7 Mouse mAb (Ab350659)?

The PSMD7 Mouse mAb (Ab350659) is formulated in PBS (Phosphate-Buffered Saline) only. It does not contain additional stabilizers such as glycerol or BSA unless specified on the lot-specific datasheet. Researchers planning long-term storage should consider adding a cryoprotectant and aliquoting the antibody.

What are the recommended storage conditions for PSMD7 Mouse mAb?

For short-term use, store the antibody at 4°C for up to one month. For long-term storage, aliquot and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles to prevent protein aggregation and loss of activity. Protect from light and avoid frost-free freezers with temperature cycling.

What research applications is PSMD7 Mouse mAb validated for?

PSMD7 Mouse mAb is typically used in applications such as Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), and Flow Cytometry (FC). Researchers should verify confirmed applications and recommended dilutions on the product datasheet, and validate the antibody in their own experimental system.

What is PSMD7 and why is it a relevant research target?

PSMD7 is a non-ATPase regulatory subunit of the 19S regulatory particle within the 26S proteasome, a central complex for ubiquitin-mediated protein degradation in eukaryotes. It plays roles in substrate recognition and proteasome function. Research on PSMD7 is relevant to understanding protein homeostasis, cancer biology, and neurodegenerative diseases.

How do I order PSMD7 Mouse mAb from Aladdin Scientific?

You can order PSMD7 Mouse mAb using SKU Ab350659 directly through the Aladdin Scientific online catalog. For institutional purchase orders, bulk pricing, or custom inquiries, please contact the Aladdin Scientific sales or customer service team. Technical support is also available for application and troubleshooting questions.

Can PSMD7 Mouse mAb be used in species other than mouse?

While the antibody is produced in mouse (host species), its reactivity against target species depends on the epitope and must be confirmed on the product datasheet. Researchers interested in cross-reactivity with human, rat, or other species should consult the datasheet or contact Aladdin Scientific technical support before proceeding.

References

1. Aladdin Scientific Product Page: PSMD7 Mouse mAb, SKU Ab350659. Aladdin Scientific.
2. Finley D. Recognition and processing of ubiquitin-protein conjugates by the proteasome. Annu Rev Biochem. 2009;78:477-513. doi:10.1146/annurev.biochem.78.081507.101607
3. Glickman MH, Ciechanover A. The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiol Rev. 2002;82(2):373-428. doi:10.1152/physrev.00027.2001

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.