Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
In E. coli expression, induction is critical. The research-standard inducer IPTG (isopropyl β-D-thiogalactopyranoside) is favored for its efficiency, stability, and low cost. However, in GMP-grade production IPTG is tightly restricted due to cell toxicity, chemical-residue risk, and regulatory ...
Blue-white screening of bacterial colonies is a popular and effective molecular biology tool often used to detect recombinant bacteria in cloning experiments. Central to this technique is the enzymatic activity of β-galactosidase, a tetrameric enzyme encoded by the lacZ α gene in E. coli that ...
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