The side chain of proline connects back to the amino nitrogen to form a five-membered pyrrolidine ring, giving proline conformational characteristics distinct from those of most α-amino acids. Attaching a second carbocycle or heterocycle at the C3 position of proline produces a ...
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Unnatural amino acids
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
3 resources · protocols, FAQs & articles
Principles, System Types, and Experimental Application Design of Cell-Free Protein Synthesis Systems
A cell-free protein synthesis (CFPS) system is an in vitro reaction platform that uses cell lysates or reconstituted translation systems to synthesize target proteins from DNA, PCR products, or mRNA templates. This system operates independently of cell growth, transformation screening, and host ...
The real change brought by unnatural amino acids is that they can now be designed around clearly defined goals. The 20 standard amino acids provide the default set of side chains used by biological systems; unnatural amino acids, by contrast, bring new functional groups, spectroscopic readouts, ...
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Techniques often explored alongside immunological experiments.