FER Mouse mAb - Primary antibody, specific to FER, Mouse IgG1

Cat. No.: Ab103180

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse

Isotype

Mouse IgG1

Clone Number

1457CT181.12.17

Conjugation

Unconjugated

Visão geral

Mouse anti Human FER Antibody, Monoclonal (1457CT181.12.17), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/100-1/500
Protein Function:
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.

Specifications

Product Name

FER Mouse mAb - Primary antibody, specific to FER, Mouse IgG1

Sinónimos

PPP1R74 | EC 2.7.10 | EC 2.7.10.2 | fer (fps/fes related) tyrosine kinase | Fer | p94-Fer | Phosphoprotein NCP94 | Proto-oncogene c-Fer | TYK3 | TYK3phosphoprotein NCP94 | Tyrosine kinase 3 | tyrosine-protein kinase Fer | c FER antibody | Feline encephali

Especificações e pureza

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Espécie hospedeira

Mouse

Especificidade

FER

Imunogénio

Recombinant FER expressed in E.coli (AA 1-300).

Controlo positivo

WB: MCF7, A549, Hep G2 and NIH/3T3 cell lysates. IHC: Human liver tissue.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein G purified

Concentração

0.5 mg/mL

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Imagens

Western Blot: FER Mouse mAb (Ab103180)

FER Mouse mAb (Ab103180) - Western Blot
All lanes: FER Mouse mAb (Ab103180) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 94 kDa
Observed band size: 93 kDa
Exposure time: 168.2 s

Western Blot: FER Mouse mAb (Ab103180)

FER Mouse mAb (Ab103180) - Western Blot
All lanes: FER Mouse mAb (Ab103180) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 94 kDa
Observed band size: 93 kDa
Exposure time: 30.0 s

IHC: FER Mouse mAb (Ab103180)

FER Mouse mAb (Ab103180) - IHC
FER Mouse mAb (Ab103180) staining FER in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Aplicação

• Aplicação: Dilution info
• IHC:

  1/100-1/500;

• WB:

  1/1000 ;

Alvos associados (humanos)

FER Tclin Tyrosine-protein kinase Fer (0 Activities)

Discover Target: FER

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (FER Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.