Grp75 Antibody - Primary antibody, specific to Grp75, Rabbit IgG

Cat. No.: Ab106524

GRADE & PURITY Carrier Free, ExactAb™, Validated, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human Grp75 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/1000 IHC: 1/50-1/100

Protein Function

Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.

Specifications

Product Name

Grp75 Antibody - Primary antibody, specific to Grp75, Rabbit IgG

Sinónimos

75 kDa glucose regulated protein antibody | 75 kDa glucose-regulated protein antibody | CSA antibody | Glucose Regulated Protein antibody | Grp 75 antibody | GRP-75 antibody | GRP75 antibody | GRP75_HUMAN antibody | Heat shock 70 kDa protein 9 antibody |

Especificações e pureza

ExactAb™, Validated, Carrier Free, 0.6 mg/mL

Espécie hospedeira

Rabbit

Especificidade

Grp75

Imunogénio

A synthetic peptide derived from human HSPA9 (AA 114-157).

Controlo positivo

WB: A549, MCF-7, HEK293, HeLa, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, Validated

Isótipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

0.6 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Grp75 Antibody (Ab106524)

Grp75 Antibody (Ab106524) - Western Blot
All lanes: Grp75 Antibody (Ab106524) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 75 kDa
Observed band size: 74 kDa
Exposure time: 4.5 s

IHC: Grp75 Antibody (Ab106524)

Grp75 Antibody (Ab106524) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using Grp75 Antibody (Ab106524). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Grp75 Antibody (Ab106524)

Grp75 Antibody (Ab106524) - IF/ICC

IF analysis of Grp75 Antibody (Ab106524) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Grp75 Antibody (Ab106524) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Aplicação

Aplicação

Dilution info

WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/200

Alvos associados (humanos)

HSPA9 Tchem Stress-70 protein, mitochondrial (0 Activities)

Discover Target: HSPA9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Grp75 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.