Hck Mouse mAb - Primary antibody, specific to Hck, Mouse IgG1

Cat. No.: Ab106982

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

1508CT602.13.1

Conjugation

Unconjugated

Visão geral

Mouse anti Human Hck Antibody, Monoclonal (1508CT602.13.1), could be used for WB, ICC, IF and so on.
Application
WB: 1/2000-1/4000
ICC/IF: 1/25
Protein Function
May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.

Specifications

Product Name

Hck Mouse mAb - Primary antibody, specific to Hck, Mouse IgG1

Sinónimos

AIPCV | p59Hck | p61Hck | Bmk | EC 2.7.10 | EC 2.7.10.2 | Hck | Hctk | hemopoietic cell kinaseJTK9 | JTK9 | p59-HCK/p60-HCK | tyrosine-protein kinase HCK | Bmk antibody | Hck 1 antibody | Hck antibody | HCK_HUMAN antibody | Hemopoietic cell kinase antibod

Especificações e pureza

ExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL

Espécie hospedeira

Mouse

Especificidade

Hck

Imunogénio

Recombinant human HCK expressed in E.coli (AA 1-526).

Controlo positivo

WB: THP-1 and Hep G2 cell lysates. ICC/IF: Hep G2 cells.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, High Performance, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein G purified

Concentração

0.5 mg/mL

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Hck Mouse mAb (Ab106982)

Hck Mouse mAb (Ab106982) - Western Blot
All lanes: Hck Mouse mAb (Ab106982) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 60 kDa
Observed band size: 58 kDa
Exposure time: 10.0 s

Western Blot: Hck Mouse mAb (Ab106982)

Hck Mouse mAb (Ab106982) - Western Blot
All lanes: Hck Mouse mAb (Ab106982) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 60 kDa
Observed band size: 58 kDa
Exposure time: 60.0 s

IF/ICC: Hck Mouse mAb (Ab106982)

Hck Mouse mAb (Ab106982) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hep G2 (human liver hepatocellular carcinoma cell line) cells labeling HCK with Hck Mouse mAb (Ab106982) at 1/25 dilution,
followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Aplicação

• Aplicação: Dilution info
• IF/ICC:

  1:25;

• WB:

  1/2000-1/4000;

• WB:

  1/2000-1/4000;

Alvos associados (humanos)

HCK Tclin Tyrosine-protein kinase HCK (0 Activities)

Discover Target: HCK

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Hck Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.