Hsp70 Mouse mAb - Primary antibody, specific to HSPA1A, Mouse IgG2b

Cat. No.: Ab108897

GRAU & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2b

Clone Number

2#

Conjugation

Unconjugated

Visão geral

Mouse anti Human Hsp70 Antibody, Monoclonal (2#), could be used for WB, IHC, ICC, IF and so on.

Application:

WB: 0.01-3µg/mL IHC: 5-20µg/mL ICC/IF: 5-20µg/mL

Protein Function

In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.Tissue specificity: HSPA1B is testis-specific.

Especificações

Product Name

Hsp70 Mouse mAb - Primary antibody, specific to HSPA1A, Mouse IgG2b

Sinónimos

DnaK type molecular chaperone HSP70 1 antibody | Epididymis secretory protein Li 103 antibody | FLJ54303 antibody | FLJ54370 antibody | FLJ54392 antibody | FLJ54408 antibody | FLJ75127 antibody | Heat shock 70 kDa protein 1 antibody | Heat shock 70 kDa pr

Especificações e pureza

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Espécie hospedeira

Mouse

Especificidade

HSPA1A

Imunogénio

Recombinant HSPA1A expressed in E.coli (AA 1-641)

Controlo positivo

WB: Hep G2, HeLa, Jurkat and A549 cell lysates. IHC: Human breast cancer, prostate tissues. ICC/IF: Hep G2 cells.

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isótipo

Mouse IgG2b

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A + Protein G purified

Concentração

See COA

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Hsp70 Mouse mAb (Ab108897)

Hsp70 Mouse mAb (Ab108897) - Western Blot
All lanes: Hsp70 Mouse mAb (Ab108897) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 0.8 s

IF/ICC: Hsp70 Mouse mAb (Ab108897)

Hsp70 Mouse mAb (Ab108897) - IF/ICC
FITC staining on IF
Samples: Human Hep G2 cell
Primary Ab: 20 μg/mL Hsp70 Mouse mAb (Ab108897)
Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Hsp70 Mouse mAb (Ab108897)

Hsp70 Mouse mAb (Ab108897) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 20 µg/mL Hsp70 Mouse mAb (Ab108897)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Hsp70 Mouse mAb (Ab108897)

Hsp70 Mouse mAb (Ab108897) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 20 µg/mL Hsp70 Mouse mAb (Ab108897)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Aplicação

Aplicação

Dilution info

WB

0.01-3 µg/mL

IHC

5-20 µg/mL

IF/ICC

5-20 µg/mL

Alvos associados (humanos)

HSPA1A Tchem Heat shock 70 kDa protein 1A (0 Activities)

Discover Target: HSPA1A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HSPA1A Tchem Heat shock 70 kDa protein 1 (164 Activities)

Discover Target: HSPA1A

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Hsp70 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.