IL-6 Mouse mAb - Primary antibody, specific to Human IL-6

Cat. No.: Ab168996

GRAU & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 0.5 mg/mL

Application

Detection Antibody, ELISA

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

C01/5B11

Conjugation

Unconjugated

Visão geral

Mouse anti Human IL-6 Antibody, clone C01/5B11, could be used for a Detection Antibody in sandwich ELISAs

Especificações

Product Name

IL-6 Mouse mAb - Primary antibody, specific to Human IL-6

Sinónimos

Interleukin BSF 2 antibody | B cell differentiation factor antibody | B cell stimulatory factor 2 antibody | B-cell stimulatory factor 2 antibody | BSF 2 antibody | BSF-2 antibody | BSF2 antibody | CDF antibody | CTL differentiation factor antibody | Cyto

Especificações e pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),0.5 mg/mL

Mecanismos bioquímicos e fisiológicos

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induc

Espécie hospedeira

Mouse

Especificidade

Human IL-6

Imunogénio

Recombinant protein corresponding to Human IL-6

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Pares emparelhados

[Ab188014] IL-6 Mouse mAb, Capture Antibody

Concentração

0.5 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imagens

Sandwich ELISA - IL-6 Antibody Pair (Ab188014 & Ab168996)

Sandwich ELISA - IL-6 standard curve
Sandwich ELISA detection of Recombinant Human IL-6 Protein (rp156005) using IL-6 Mouse mAb (Ab188014) as capture antibody at concentration of 1.0 µg/mL. Biotinylated IL-6 Mouse mAb (Ab168996) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 10.0 ng/mL - 10000.0 ng/mL 

Indirect ELISA - IL-6 Mouse mAb (Ab168996)

Indirect ELISA - IL-6 standard curve
Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with IL-6 Mouse mAb (Ab168996). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC₅₀: 23.14 ng/mL

Aplicação

Aplicação

Dilution info

ELISA

1.0 µg/mL as a Detection antibody in ELISA

Alvos associados (humanos)

IL6 Tclin Interleukin-6 (0 Activities)

Discover Target: IL6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IL-6 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.