Iscalimab (anti-CD40) - Primary antibody, specific to CD40, Human IgG1, Antibody of CD40, Antibody of CD40

CAS: 2031153-61-2 Cat. No.: Ab175491

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE&SEC-HPLC)

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Human

Reactive species

Cynomolgus monkey, Human, Rhesus monkey

Isotype

Human IgG1

Conjugation

Unconjugated

Visão geral

Iscalimab (anti-CD40) is a non-depleting IGg1 monoclonal antibody targeting CD40 (KD: 0.3 nM). Iscalimab (anti-CD40) can be used for research of Graves' hyperthyroidism and autoimmune diseases.
Purity
≥95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Specifications

Product Name

Iscalimab (anti-CD40) - Primary antibody, specific to CD40, Human IgG1, Antibody of CD40

Sinónimos

AI326936 antibody | B cell associated molecule CD40 antibody | B cell surface antigen CD40 antibody | B cell-associated molecule antibody | B-cell surface antigen CD40 antibody | Bp50 antibody | CD 40 antibody | CD40 antibody | CD40 antigen (TNF receptor

Especificações e pureza

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC)

Espécie hospedeira

Human

Especificidade

CD40

Conjugação

Unconjugated

Grau

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Tipo de ação

INHIBITOR

Mecanismo de ação

Antibody of CD40

Isótipo

Human IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

27.2 kDa (Light Chain) & 52.2 kDa (Heavy Chain), under reducing conditions; 201.2 kDa, under non-reducing conditions.

Método de purificação

Protein A purified

CAS

2031153-61-2

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -80°C,Avoid repeated freezing and thawing

Enviado em

Dry ice packs + Cold packs

Estabilidade e armazenamento

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Flow Cytometry: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 with Iscalimab (anti-CD40) (Ab175491) conjugated with biotin (right panel) compared with Human IgG (Biotin) (Ab180314) - Isotype Control (left panel) and detected with SA-PE (rp156150). 

ELISA: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - ELISA
Immobilized Recombinant Human CD40 protein (rp183577) at 1.0 μg/mL can bind Iscalimab (anti-CD40) (Ab175491) with the EC₅₀ of 35.88 ng/mL.

SEC: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - SEC
The purity of Iscalimab (anti-CD40) (Ab175491) is more than 95% verified by HPLC.

Flow Cytometry: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD40 (red) with Iscalimab (anti-CD40) (Ab175491). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 (red) with Iscalimab (anti-CD40) (Ab175491) conjugated with biotin and detected with SA-PE (rp156150). Blue - Isotype control, Human IgG (Biotin) (Ab180314). Black - Unlabelled control, cells without incubation with primary antibody.

Alvos associados (humanos)

CD40 Tchem Tumor necrosis factor receptor superfamily member 5 (0 Activities)

Discover Target: CD40

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD40 Tchem Tumor necrosis factor receptor superfamily member 5 (9 Activities)

Discover Target: CD40

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de ação

Perguntas frequentes

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2031153-61-2.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Iscalimab (anti-CD40)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.