Goat Anti-Human IgG (PE) - Secondary antibody

Cat. No.: Ab175838
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Ex:565nm ? Fluorescence excitation maximum at 565 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:575nm ? Fluorescence emission maximum at 575 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 1.0 mg/mL
Application
Flow Cytometry, IF/ICC
Host species
Goat
Reactive species
Human
Conjugation
PE
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
100μg
Ab175838-100μg
5
$99.90
1mg
Ab175838-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$719.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Validated, Azide Free, Ex:565nm, Em:575nm, 1.0 mg/mL Azide Free,Em:575nm,Ex:565nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing Ships Wet ice Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application
Flow: 1:100-1:1000
IF: 1:1000

Specifications

Product Name
Goat Anti-Human IgG (PE) - Secondary antibody
Synonyms
Goat Anti-Human Antibody (PE) | PE-conjugated Goat Anti-Human Antibody | Goat Anti-Human IgG / PE antibody | Goat Anti-Human IgG-PE antibody
Specifications & Purity
ExactAb™, Validated, Azide Free, Ex:565nm, Em:575nm, 1.0 mg/mL
Host species
Goat
Conjugation
PE
Excitation (nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
Grade
Azide Free, Em:575nm, Ex:565nm, ExactAb™, Validated
Purification Method
Antigen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In
Wet ice
Stability And Storage
Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles.
Images

Goat Anti-Human IgG (PE) (Ab175838) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Rituximab (anti-CD20) (R412001). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/100 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
IF/ICC

1:1000

Flow Cytometry

1/100-1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001392Certificate of AnalysisMar 20, 2026 Ab175838
ZJ23F1001391Certificate of AnalysisJul 30, 2024 Ab175838
Documents & Articles
Immunocytochemistry (ICC) protocol
RNA dot blot protocol
Caspase Immunofluorescence Staining Protocol
General Protocol for Western Blotting
Western Blotting with Fluorescently Labeled Antibodies
Guidelines of Secondary Antibody for Western Blotting
Flow Cytometry Using Methanol Permeabilization
CLARITY Tissue Clearing Staining Technology
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View Ex:565nm grade guide → View Em:575nm grade guide →

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