Letaplimab (anti-CD47) - Primary antibody, specific to CD47, Human IgG4SP

CAS: 2283356-07-8 Cat. No.: Ab190155

GRAU & PUREZA Animal Free, Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Human

Reactive species

Cynomolgus monkey, Human

Isotype

Human IgG4SP

Conjugation

Unconjugated

Visão geral

Letaplimab (anti-CD47) is a human IgG4SP anti-CD47 monoclonal antibody. CD47 is overexpressed on cancer cells, it is a ligand for SIRPα. Letaplimab (anti-CD47) enhances tumor cell phagocytosis.
Purity
≥95% (SDS-PAGE&SEC)
Endotoxin Level
≤1.0EU/mg
Protein Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Especificações

Product Name

Letaplimab (anti-CD47) - Primary antibody, specific to CD47, Human IgG4SP

Sinónimos

Antigen identified by monoclonal antibody 1D8 antibody | Antigenic surface determinant protein OA3 antibody | CD 47 antibody | CD47 antibody | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody | CD47 antigen antibody | CD47

Especificações e pureza

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Espécie hospedeira

Human

Especificidade

CD47

Conjugação

Unconjugated

Grau

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Isótipo

Human IgG4SP

Tipo de corrente de luz

kappa

SDS-PAGE

142.38 kDa

Método de purificação

Protein A purified

Concentração

See COA

CAS

2283356-07-8

Product Line

Produtos biológicos

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at -80°C,Avoid repeated freezing and thawing

Enviado em

Dry ice packs + Cold packs

Estabilidade e armazenamento

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Flow Cytometry: Letaplimab (anti-CD47) (Ab190155)

Letaplimab (anti-CD47) (Ab190155) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling CD47 (red) with Letaplimab (anti-CD47) (Ab190155) at 1.0 μg/mL for 1 hour at 4°C. Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

ELISA: Letaplimab (anti-CD47) (Ab190155)

Letaplimab (anti-CD47) (Ab190155) - ELISA
Immobilized Recombinant Human CD47 protein (rp144028) at 1.0 μg/mL can bind Letaplimab (anti-CD47) (Ab190155) with the EC ₅₀  of 182.6 ng/mL.

SEC: Letaplimab (anti-CD47) (Ab190155)

Letaplimab (anti-CD47) (Ab190155) - SEC
The purity of Letaplimab (anti-CD47) (Ab190155) is more than 95% verified by HPLC.

Alvos associados (humanos)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)

Discover Target: CD47

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2283356-07-8.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Letaplimab (anti-CD47)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.