MeCP2 Mouse mAb - Primary antibody, specific to MECP2, Mouse IgG1

Cat. No.: Ab114749

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, See COA

Application

WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

C7

Conjugation

Unconjugated

Visão geral

Mouse anti Human MeCP2 Antibody, Monoclonal (C7), could be used for WB and so on.
Application:
WB: 0.5-2µg/mL
Protein Function
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.
Post-translational
Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.

Specifications

Product Name

MeCP2 Mouse mAb - Primary antibody, specific to MECP2, Mouse IgG1

Sinónimos

AUTSX 3 antibody | AUTSX3 antibody | DKFZp686A24160 antibody | Mbd 5 antibody | Mbd5 antibody | MECP 2 antibody | MeCP 2 protein antibody | MeCP-2 protein antibody | Mecp2 antibody | MECP2_HUMAN antibody | Methyl CpG binding protein 2 (Rett syndrome) anti

Especificações e pureza

Carrier Free, ExactAb™, Azide Free, Validated, See COA

Espécie hospedeira

Mouse

Especificidade

MECP2

Imunogénio

Recombinant MECP2 expressed in E.coli (AA 1-177).

Controlo positivo

WB: SK-N-SH, NIH/3T3 and C6 cell lysates.

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A + Protein G purified

Concentração

See COA

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 2-8°C short term (2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: MeCP2 Mouse mAb (Ab114749)

MeCP2 Mouse mAb (Ab114749) - Western Blot
All lanes: MeCP2 Mouse mAb (Ab114749) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 81 kDa
Exposure time: 60.0 s

Western Blot: MeCP2 Mouse mAb (Ab114749)

MeCP2 Mouse mAb (Ab114749) - Western Blot
All lanes: MeCP2 Mouse mAb (Ab114749) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 51 kDa
Exposure time: 52.9 s

Aplicação

• Aplicação: Dilution info
• WB:

  0.5-2 µg/mL;

Alvos associados (humanos)

MECP2 Tchem Methyl-CpG-binding protein 2 (0 Activities)

Discover Target: MECP2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MeCP2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.