Recombinant Caspr Antibody - Primary antibody, specific to CNTNAP1, Rabbit IgG

Cat. No.: Ab093095

GRADE & PURITY Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human Caspr Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name

Recombinant Caspr Antibody - Primary antibody, specific to CNTNAP1, Rabbit IgG

Sinónimos

Caspr antibody | Caspr1 antibody | CNTNAP antibody | Cntnap1 antibody | CNTP1_HUMAN antibody | Contactin associated protein 1 antibody | Contactin-associated protein 1 antibody | MHDNIV antibody | NCP1 antibody | Neurexin 4 antibody | Neurexin IV antibody

Especificações e pureza

Recombinant, ExactAb™, Validated, See COA

Mecanismos bioquímicos e fisiológicos

Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have

Espécie hospedeira

Rabbit

Especificidade

CNTNAP1

Imunogénio

A synthetic peptide derived from human Caspr (AA 700-770)

Controlo positivo

WB: HeLa cell lysates.

Conjugação

Unconjugated

Grau

ExactAb™, Recombinant, Validated

Isótipo

Rabbit IgG

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

See COA

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant Caspr Antibody (Ab093095)

Recombinant Caspr Antibody (Ab093095) - Western Blot
All lanes: Recombinant Caspr Antibody (Ab093095) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 156 kDa
Observed band size: 206 kDa
Exposure time: 80 s

Aplicação

Aplicação

Dilution info

WB

1/1000 - 1/2000

Alvos associados (humanos)

CNTNAP1 Tbio Contactin-associated protein 1 (0 Activities)

Discover Target: CNTNAP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

• Tested applications, recommended dilutions, and positive controls: Not specified for this item; refer to CoA/Spec Sheet.
• General notes (non-item-specific): Application performance can vary by fixation, retrieval, blocking, and detection system. Optimize antibody dilution, incubation time/temperature, and wash conditions empirically on your samples.

Biological Roles

• Target overview (literature/general):
  • Caspr (Contactin-associated protein 1; CNTNAP1) is a transmembrane cell-adhesion/scaffolding protein of the neurexin superfamily. It concentrates at paranodal junctions between axons and myelinating glia, partnering with Contactin to form septate-like junctions.
  • Functions include maintaining axo‑glial junction integrity, organizing paranodal domains, and segregating voltage-gated ion channels along myelinated axons. This compartmentalization is essential for saltatory conduction.
  • Caspr’s extracellular domains mediate adhesion; its intracellular tail interacts with cytoskeletal adaptors (e.g., 4.1 family), anchoring paranodal complexes.
• Research relevance (general, non-clinical):
  • Antibodies to Caspr are widely used to visualize paranodes in rodent and human tissues, assess myelination status, and study nodal/paranodal remodeling during development or injury.
  • In cellular and tissue studies, co-staining with nodal (e.g., Nav channels) and juxtaparanodal (e.g., Kv1 channels) markers helps map axonal domains and quantify structural integrity.
• Caution: The above describes the biology of the target protein and typical research uses. It does not imply or describe validated applications, species reactivity, or performance for this specific antibody lot, which are Not specified for this item; refer to CoA/Spec Sheet.

Buffer Applications

• This product itself is not a buffer component. However, antibodies are typically handled in standard biological buffers.
• General guidance (literature):
  • Common assay buffers: PBS or TBS at pH ~7.2–7.6. For immunostaining, include mild detergent (e.g., 0.05–0.1% Tween‑20) for washes; for blocking, use serum, BSA, or casein as appropriate.
  • Antigen retrieval for IHC on formalin-fixed paraffin-embedded tissues may require citrate or Tris‑EDTA buffers; conditions must be optimized per tissue and epitope.
  • Low-protein-binding tubes and filtered buffers reduce background and loss. Maintain isotonicity and avoid microbial contamination (filter-sterilize; include preservatives if compatible).
• Item-specific formulation and recommended working buffer are Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

• Green chemistry substitution is not directly applicable to antibody reagents.
• Sustainability considerations (general):
  • Recombinant production can reduce reliance on animal-based hybridoma systems, aligning with 3Rs principles (replacement, reduction, refinement).
  • Minimize cold-chain footprint by consolidating shipments and using aliquots to avoid waste from multiple freeze–thaw cycles.
  • Choose aqueous buffer systems and avoid unnecessary organic solvent use in workflows.

Pharmaceutical Uses

• This product is supplied for research use only (per Product Data). It is not intended for human or veterinary use, diagnostic procedures, or as an API/excipient.
• General context (non-clinical):
  • Recombinant antibodies may be used as analytical reagents in bioprocess development (e.g., ligand in capture assays) and exploratory diagnostics research. Any GMP or clinical application would require appropriate qualification and is outside the scope of this product.
• Regulatory note: For regulated workflows, request supporting documentation (sequence, impurity profile, host cell protein/DNA testing) if available. For this item, such details are Not specified; refer to CoA/Spec Sheet.

Physical Properties

• Item-specific values:
  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Concentration, formulation buffer, and additives (e.g., glycerol, BSA, preservatives): Not specified for this item; refer to CoA/Spec Sheet.
• Storage Conditions (from Product Data): Store at -20°C. Avoid repeated freezing and thawing.
• Shipping (from Product Data): Ice chest + ice pads.
• Hazard physico-chemical data: Not specified for this item; refer to SDS.
• General/literature notes for antibodies (not item-specific):
  • Typical IgG molecular mass ~150 kDa; Fab ~50 kDa; scFv ~25–30 kDa. Recombinant formats (IgG, Fab, scFv, VHH) vary widely.
  • Isoelectric point (pI) commonly ~6–9 depending on sequence and glycosylation.
  • Solubility: Antibodies are water-soluble in buffered aqueous media (e.g., PBS, Tris) with stability enhanced by isotonic salts, sugars, and polyols.
  • Stability is influenced by pH (often optimal near neutral), ionic strength, and freeze–thaw history. Repeated freeze–thaw cycles can cause aggregation/fragmentation.
• Refractive index, logP, pKa: Not applicable/meaningful for intact proteins in the classic small-molecule sense.

Quality and Grades

• Item-specific grade statements (from Product Data):
  • Recombinant: Produced by recombinant expression for defined sequence and enhanced lot-to-lot consistency compared with hybridoma-derived antibodies.
  • ExactAb™: Branded designation indicating precise, sequence-defined recombinant antibody production and control. This typically implies traceable sequence, reproducible manufacture, and minimized batch variability. Specific platform details may be described in the CoA/Spec Sheet.
  • Validated: Indicates the antibody has undergone application/target validation by the manufacturer. Exact tested applications, sample types, and validation data are Not specified for this item; refer to CoA/Spec Sheet.
• Practical implications for users (general):
  • Recombinant format reduces drift in affinity and specificity across lots, enabling reliable longitudinal studies and quantitative methods.
  • Validation status should be matched to your intended application (e.g., WB, IHC, IF, IP). Request the validation images/metrics for your assay conditions.
  • If stabilizers (e.g., BSA, trehalose, glycerol, azide) are present, they impact downstream uses (e.g., MS compatibility, live-cell staining, conjugation). Composition is Not specified for this item; check CoA/Spec Sheet.
• Documentation:
  • For method qualification, maintain vendor CoA, SDS, and any sequence/citation sheets associated with the ExactAb™ lot.

Reaction and Applications

• Not applicable in the synthetic chemistry sense. As a biological affinity reagent, this antibody is used for antigen detection/capture rather than participating as a reactant or catalyst.
• Relevant use context (general): immunoassays (WB, IHC, IF, ELISA), immunoprecipitation, and imaging where the target is Caspr (CNTNAP1). Specific tested applications for this item are Not specified; refer to CoA/Spec Sheet.

Reaction Conditions

• Not applicable to chemical reaction conditions. The antibody functions as an affinity reagent rather than a reactant or catalyst.
• Assay conditions (general, non-item-specific): Typical immunoassay workflows use near‑neutral aqueous buffers, controlled temperature (room temperature to 4°C), and gentle mixing to preserve protein integrity. Specific conditions for this item are Not specified; refer to CoA/Spec Sheet.

Safety and Handling

• Classification and hazards (item-specific):
  • GHS Classification, Signal Word, H-statements, Pictograms: Not specified for this item; refer to SDS.
• General safety for protein antibodies (literature/general):
  • Typically considered low hazard for laboratory handling; however, treat as a proteinaceous biological reagent that can cause allergic reactions in sensitized individuals.
  • Avoid ingestion, inhalation of aerosols, and contact with eyes/skin. Prevent microbial contamination.
• Recommended PPE and engineering controls:
  • Wear lab coat, disposable nitrile gloves, and safety glasses. Handle in a clean area or biosafety cabinet if aerosol generation is possible.
  • Use low-protein-binding tips/tubes to minimize loss and avoid generating aerosols during pipetting or vortexing.
• Incompatibilities and stability considerations:
  • Avoid extreme pH, high shear, and organic solvents; these can denature antibodies.
  • Prevent repeated freeze–thaw cycles (per Product Data). Aliquot upon first thaw.
• First-aid (general guidance; defer to SDS):
  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing.
  • Inhalation: Move to fresh air.
  • Ingestion: Rinse mouth; seek medical advice.
• Disposal: Follow institutional guidelines for non-hazardous biological/chemical waste and local regulations.

Solvent Selection

• Not typically applicable. This product is a recombinant antibody intended for use in aqueous biological buffers rather than organic solvents.
• General guidance (literature):
  • Common working buffers: PBS, TBS, or Tris-based buffers at near-neutral pH. Additives such as 0.01–0.1% Tween-20 can reduce nonspecific binding in immunoassays; sugars or polyols may enhance stability for storage.
  • Avoid strong denaturants, chaotropes, or organic solvents unless intentionally performing SDS-PAGE or denaturing protocols.

Storage and Reconstitution

• Item-specific storage (from Product Data): Store at -20°C. Avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads (from Product Data).
• Reconstitution and handling: Not specified for this item; refer to CoA/Spec Sheet.
• Practical handling guidance (general, literature):
  • Upon receipt, if the antibody is lyophilized, reconstitute with sterile, nuclease-free water or buffer as recommended on the CoA, then mix gently and allow full dissolution on ice.
  • If supplied in solution, keep on ice during use. Aliquot into low-protein-binding tubes to avoid repeated freeze–thaw cycles. Store working aliquots at -20°C or below; for long-term storage, -80°C is commonly used if compatible with formulation.
  • Avoid vigorous vortexing or foaming; gently invert to mix. Centrifuge briefly to collect liquid before opening.
  • Presence/absence of preservatives (e.g., sodium azide) or carriers (e.g., BSA, glycerol, trehalose) affects storage temperature and compatibility with live-cell applications or conjugation. Composition is Not specified for this item; consult CoA/Spec Sheet.
• Research Use Note: For research use only.

Structure and Identity

• Item-specific identifiers:
  • SKU: Ab093095
  • Product Name: Recombinant Caspr Antibody
  • Grade/Purity: Recombinant, ExactAb™, Validated (per Product Data)
• Molecular descriptors:
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES / InChI / InChIKey: Not applicable for protein biologics; not provided for this item.
• Structural features (general, literature):
  • Antibodies are Y‑shaped immunoglobulins composed of two heavy and two light chains linked by disulfide bonds, with variable domains forming the antigen-binding sites (paratopes) and constant domains determining isotype and Fc effector properties.
  • “Recombinant” indicates sequence-defined expression in a controlled host system (e.g., mammalian or yeast) rather than hybridoma-derived, enabling lot-to-lot consistency.
• Target overview (general):
  • Caspr (Contactin‑associated protein 1; CNTNAP1) is a neuronal cell-adhesion/scaffold protein localized at paranodal junctions of myelinated axons. Antibodies against Caspr are commonly used to delineate paranodes in neurobiology studies.
• Isotype/clone/host: Not specified for this item; refer to CoA/Spec Sheet.
• Conjugation: Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

• Not applicable. As a recombinant antibody, this product is not used as a synthetic building block or reagent in chemical synthesis.
• For experimental planning, please refer instead to the Biological Roles, Target Specificity, and Application Protocols sections.

Target Specificity

• Antigen/Target: Not specified for this item; refer to CoA/Spec Sheet. Note: Product name indicates Caspr, but definitive target details must be confirmed on the CoA/Spec Sheet for this lot.
• Species reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Clone ID / Isotype / Host: Not specified for this item; refer to CoA/Spec Sheet.
• Conjugate: Not specified for this item; refer to CoA/Spec Sheet.
• Epitope mapping/affinity: Not specified for this item; refer to CoA/Spec Sheet.

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Caspr Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.