Recombinant Caveolin-2 Antibody - Primary antibody, specific to CAV2, Rabbit IgG

Cat. No.: Ab093252

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.2 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human Caveolin-2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
Post-translational
Post-translational': "Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.

Specifications

Product Name

Recombinant Caveolin-2 Antibody - Primary antibody, specific to CAV2, Rabbit IgG

Sinónimos

CAV antibody | CAV2 antibody | CAV2_HUMAN antibody | Caveolae protein 20 kD antibody | Caveolin 2 antibody | Caveolin 2 isoform a and b antibody | Caveolin-2 antibody | MGC12294 antibody | OTTHUMP00000025032 antibody | OTTHUMP00000195982 antibody | CAV |

Especificações e pureza

ExactAb™, Validated, Recombinant, 0.2 mg/mL

Espécie hospedeira

Rabbit

Especificidade

CAV2

Imunogénio

A synthetic peptide derived from human Caveolin-2 (AA 14-27)

Controlo positivo

WB: HeLa, A549, SK-OV-3, U2OS and HUVEC cell lysates. IHC: Human lung cancer tissue. ICC/IF: A549 cells.

Conjugação

Unconjugated

Grau

ExactAb™, Recombinant, Validated

Isótipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificação

Antigen affinity purified

Concentração

0.2 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant Caveolin-2 Antibody (Ab093252)

Recombinant Caveolin-2 Antibody (Ab093252) - Western Blot
All lanes: Recombinant Caveolin-2 Antibody (Ab093252) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 18 kDa
Observed band size: 18, 20 kDa
Exposure time: 23.1 s

IF/ICC: Recombinant Caveolin-2 Antibody (Ab093252)

Recombinant Caveolin-2 Antibody (Ab093252) - IF/ICC
Immunocytochemistry analysis of Caveolin 2 (green) in A549 using Recombinant Caveolin-2 Antibody (Ab093252), and cells were counterstained with DAPI (blue).

IHC: Recombinant Caveolin-2 Antibody (Ab093252)

Recombinant Caveolin-2 Antibody (Ab093252) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant Caveolin-2 Antibody (Ab093252). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Aplicação

Aplicação

Dilution info

WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Alvos associados (humanos)

CAV2 Tbio Caveolin-2 (0 Activities)

Discover Target: CAV2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item-specific, tested applications and recommended dilutions are not provided for this SKU; refer to the CoA/Spec Sheet.

General protocol guidance (literature)

• Western blot: Start at 1:500–1:2,000 for primary antibody, depending on antibody concentration and detection system; use BSA for blocking to preserve phospho-sensitive epitopes if relevant.
• Immunofluorescence/Immunocytochemistry: Start at 1:200–1:1,000; include appropriate fixation (PFA) and permeabilization for intracellular targets. Co-stain with Caveolin-1 for caveolae localization studies as needed.
• Immunoprecipitation: 1–5 µg antibody per 0.5–1 mg total protein; gentle non-ionic detergents (e.g., 0.5–1% NP-40 or digitonin) help maintain caveolar complexes.
• ELISA: Coat antigen at 0.5–2 µg/mL; start primary at 0.1–1 µg/mL or 1:1,000–1:10,000 depending on formulation. These are starting points only and not specifications for this item. Optimize for antibody concentration, exposure, and sample type.

Biological Roles

General biology of the target (literature)

• Caveolin-2 (CAV2) is a membrane-associated scaffolding protein enriched in caveolae, often forming hetero-oligomers with Caveolin-1. It participates in organizing cholesterol-rich microdomains that regulate endocytosis, signal transduction, and mechanotransduction.
• Reported interactions include roles in regulating MAPK/ERK signaling, nitric oxide synthase pathways, and lipid trafficking. CAV2 subcellular distribution is primarily at the plasma membrane and Golgi-associated compartments, with tissue-specific expression.
• Post-translational features such as palmitoylation can influence membrane association and protein–protein interactions.

Relevance to research use

• Antibodies to CAV2 enable detection of caveolae dynamics, co-localization with CAV1, and interrogation of membrane domain signaling by immunoblot, immunofluorescence, and immunoprecipitation (assay suitability depends on antibody validation).

Item-specific note

• This SKU is designated against Caveolin-2 by name, but clone, epitope, species reactivity, and tested applications are not specified in the provided data. Confirm details via the product datasheet before planning experiments.

Buffer Applications

Not a buffering reagent. For experimental use, select assay-appropriate buffers to maintain antibody stability and target epitope integrity:

• Storage/working buffers (general): PBS or TBS, pH 7.2–7.6, with 0.01–0.05% Tween-20 for some immunoassays; 1–5% BSA or appropriate serum for blocking.
• For immunofluorescence: Isotonic PBS; include permeabilizers (e.g., 0.1–0.3% Triton X-100) only if the epitope is intracellular and tolerant to permeabilization.
• For immunoprecipitation: Lysis buffers matched to membrane protein targets (e.g., 1% NP-40 or digitonin), maintaining gentle conditions to preserve caveolae-associated complexes. These are general guidelines; the optimal buffer system for this item is not specified and should be verified against the CoA/Spec Sheet and internal validation.

Green Alternatives

Green chemistry substitution is not directly applicable to antibody reagents. Sustainability considerations for this item focus on:

• Cold-chain logistics: Consolidate orders to reduce shipping emissions and maintain continuous temperature control to minimize waste.
• Buffer choices: Use phosphate or HEPES buffers prepared with ultrapure water; avoid excessive single-use plastics by aliquoting into reusable, sterile screw-cap tubes where appropriate.
• Preservatives: If azide-free formulations are available and compatible with your application, they can simplify wastewater handling; confirm availability for this specific SKU in the CoA. These are general practices; product-specific greener options are not specified for this item.

Pharmaceutical Uses

This product is supplied for research use only. It is not intended for human or veterinary use, diagnostic procedures, or as a therapeutic. In pharmaceutical R&D workflows, anti-Caveolin-2 antibodies may be used as analytical reagents for target quantitation, mechanism studies, and biomarker method development in discovery or process analytics. No pharmacopeial status, GMP qualification, or clinical application is specified for this item. For regulated use, obtain documentation on lot traceability, adventitious agent control, and validation data from the manufacturer.

Physical Properties

Item-specific facts (from Product Data)

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight and formula: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, buffer composition, and additives (e.g., glycerol, BSA, azide): Not specified for this item; refer to CoA/Spec Sheet.

General properties for recombinant antibodies (literature)

• Typical IgG molecular mass: ~150 kDa; fragments vary (Fab ~50 kDa, scFv ~25–30 kDa).
• Protein solubility: Aqueous buffers (e.g., PBS, Tris) at pH 6.5–8.0; stability enhanced by isotonic salts and stabilizers such as 10–50% glycerol.
• Isoelectric point: IgGs commonly pI 6–9 (highly clone-dependent).
• Temperature sensitivity: Freeze–thaw cycles can denature and aggregate proteins; slow thaw on ice is recommended.

Notes

• Exact biophysical values (extinction coefficient, pI, aggregation state, excipients) are lot- and construct-specific and are not provided here. Consult the CoA/SDS for definitive specifications.

Quality and Grades

Item-specific facts (from Product Data)

• Grade/Purity: Recombinant, ExactAb™, Validated.

Interpretation and implications

• Recombinant: Produced from a defined genetic sequence, offering batch-to-batch consistency and elimination of animal immunization variability typical of hybridoma-derived antibodies.
• ExactAb™: Brand/designation indicating engineered, sequence-defined antibody production. Typical benefits include clonality, isotype control, and reduced lot drift. Specific isotype/subclass and sequence features are not specified here and should be confirmed in the CoA.
• Validated: Indicates the product has undergone application-specific or target-specific verification by the manufacturer. Exact tested applications, species reactivity, and validation data are not listed in this record.

What to verify for your assay

• Confirm format (full IgG vs fragment), host species/isotype, concentration, preservative/stabilizer content, and tested applications before use in quantitative workflows.
• For regulated studies, request lot-specific CoA, validation reports, and if available, sequence or clonality documentation associated with ExactAb™ products.

Reaction and Applications

Not applicable in the context of chemical synthesis. As an antibody reagent, this product is intended for biochemical detection or capture of Caveolin-2 rather than participation in chemical reactions. For practical usage, refer to the Application Protocols tab (assay workflows) and the Target Specificity tab (epitope/species), noting that specific parameters are not provided in this record and should be confirmed in the CoA or technical datasheet.

Reaction Conditions

Not applicable to chemical reactions. For assay conditions in immunoassays, only general guidance can be provided here because item-specific parameters are not supplied:

• Western blot (general): Load 10–30 µg total protein per lane; use PVDF membranes; typical primary antibody incubation 1–16 h at 4–25°C in TBST + 5% BSA.
• Immunofluorescence (general): Fixation with 4% PFA; permeabilize with 0.1–0.3% Triton X-100 if needed; block with 3–5% BSA; primary incubation 1–2 h at room temp or overnight at 4°C.
• Immunoprecipitation (general): 1–5 µg antibody per 0.5–1 mg lysate protein; incubate 1–4 h at 4°C; capture with Protein A/G beads. These are literature-style norms and are not specifications for this item. Optimize empirically and consult the product datasheet when available.

Safety and Handling

Item-specific facts (from Product Data)

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• Shipping: Ice chest + ice pads (cold chain).
• Storage: Store at −20°C; avoid repeated freezing and thawing.

General safety guidance for antibody reagents (literature)

• PPE: Laboratory coat, gloves, and eye protection. Handle as a protein solution potentially containing preservatives (e.g., sodium azide) unless otherwise specified.
• Incompatibilities: Avoid strong oxidizers and proteases; minimize exposure to elevated temperatures and extreme pH that can denature proteins.
• Biological origin risk: Recombinant antibodies are non-infectious; nonetheless, treat as low-hazard biologicals. If sodium azide is present, avoid contact with metals in plumbing due to formation of explosive metal azides.
• First aid (general): Skin/eye contact—rinse with water. Inhalation of sprays—move to fresh air. Ingestion—rinse mouth. Seek medical advice if irritation persists.

Always consult the product-specific SDS and CoA for authoritative hazard classifications, preservative content, and disposal instructions.

Solvent Selection

Not typically applicable. This product is a recombinant antibody supplied in an aqueous buffer. Use standard biological buffers (e.g., PBS or Tris) compatible with your assay. If lyophilized or concentrated, reconstitute/dilute with the recommended buffer from the CoA/Spec Sheet. Avoid organic solvents that denature proteins unless specifically required for specialized applications.

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage conditions: Store at −20°C. Avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads (maintain cold chain).

General guidance (literature)

• Upon receipt: Inspect for any signs of thaw or leakage. If liquid, mix gently by inversion; avoid vortexing.
• Aliquoting: Immediately divide into single-use aliquots to prevent freeze–thaw cycles. Use low-protein-binding tubes.
• Thawing: Thaw on ice; keep at 2–8°C during short-term use. Do not refreeze multiple times.
• Reconstitution (if lyophilized; not specified for this item): Reconstitute in sterile PBS or recommended buffer to the desired concentration; allow gentle mixing at room temperature for 10–15 minutes.
• Short-term storage: 2–8°C for up to a few days if formulation permits (verify preservative content). For longer-term storage, keep at −20°C or below, per product data; some formats tolerate −80°C for extended periods.
• Stabilizers: Addition of 10–50% glycerol can improve freeze–thaw stability for many antibody formats; confirm compatibility with your assay and the specific formulation. Always defer to the product’s CoA/Spec Sheet for definitive formulation and storage instructions for this SKU.

Structure and Identity

Item-specific facts (from Product Data)

• Product: Recombinant Caveolin-2 Antibody (SKU: Ab093252); category: Antibody; format: Recombinant, ExactAb™, Validated.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChI / InChIKey: Not applicable to proteins; not specified for this item.

General structural description (literature)

• Antibodies are Y-shaped glycoproteins composed of two heavy and two light chains stabilized by disulfide bonds, forming variable (V) and constant (C) domains. The antigen-binding sites reside in the VH/VL complementarity determining regions (CDRs).
• Common research formats include full-length IgG (~150 kDa), recombinant fragments (Fab ~50 kDa; scFv ~25–30 kDa), or engineered Fc fusions. Exact format for this product is not specified in the data provided.
• Target: Caveolin-2 (CAV2), a caveolae-associated scaffolding protein. Epitope mapping, isotype, and species reactivity are not provided in the current record.

Synthetic Utility

Not applicable. As a recombinant antibody, this product is not a chemical building block and is not used in organic synthesis. Its utility lies in biochemical detection, capture, or modulation of the Caveolin-2 protein in research assays. Refer to Application Protocols for assay-oriented guidance (details for this SKU are not specified in the provided data).

Target Specificity

Item-specific facts (from Product Data)

• Designation: Recombinant Caveolin-2 Antibody.
• Detailed clone ID, isotype/subclass, epitope/peptide, species reactivity (human/mouse/rat/others), cross-reactivity, and applications validated: Not specified for this item; refer to CoA/Spec Sheet.

General notes (literature)

• Caveolin-2 (CAV2) sequence conservation varies by species; cross-reactivity depends on epitope selection and antibody format. Validation should include positive/negative controls such as CAV2-expressing cell lines or tissues and CAV2 knockout/siRNA samples where available.

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Caveolin-2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.