Recombinant Caveolin-3 Antibody - Primary antibody, specific to CAV3, Rabbit IgG

Cat. No.: Ab093262

GRAU & PUREZA Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human Caveolin-3 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.

Especificações

Product Name

Recombinant Caveolin-3 Antibody - Primary antibody, specific to CAV3, Rabbit IgG

Sinónimos

CAV3 | caveolin 3 | Caveolin3 | Caveolin-3 | LGMD1C | LGMD1CMGC126100 | LQT9 | LQT9MGC126101 | M-caveolin | MGC126129 | VIP21 | VIP-21 | CAV3 antibody | CAV3_HUMAN antibody | Caveolin 3 antibody | Caveolin-3 antibody | LGMD1C antibody | LQT9 antibody | M-

Especificações e pureza

Recombinant, ExactAb™, Validated, See COA

Espécie hospedeira

Rabbit

Especificidade

CAV3

Imunogénio

A synthetic peptide derived from human Caveolin-3 (AA 1-60)

Controlo positivo

WB: Mouse heart and Rat heart lysates.

Conjugação

Unconjugated

Grau

ExactAb™, Recombinant, Validated

Isótipo

Rabbit IgG

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

See COA

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant Caveolin-3 Antibody (Ab093262)

Recombinant Caveolin-3 Antibody (Ab093262) - Western Blot
All lanes: Recombinant Caveolin-3 Antibody (Ab093262) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 19 kDa
Exposure time: 3.7 s

Aplicação

Aplicação

Dilution info

WB

1/500-1/2000

Alvos associados (humanos)

CAV3 Tbio Caveolin-3 (0 Activities)

Discover Target: CAV3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Manufacturer-provided, item-specific application details are not specified; consult the CoA/Spec Sheet. The following are general starting protocols for anti-Caveolin-3 antibodies and must be optimized by the user.

• Western blot (general):

  • Sample: Muscle tissue lysates or CAV3-expressing cells; use RIPA or membrane-enriching lysis buffers with protease inhibitors.
  • Load: 10–40 µg total protein per lane. Transfer to PVDF. Block in 3–5% BSA/TBST.
  • Primary: Start at 1:1,000 in BSA/TBST; incubate overnight at 4°C. Adjust based on signal-to-noise.
  • Secondary: HRP-conjugated anti-species IgG; detect via ECL.

• Immunofluorescence (general):

  • Fix: 4% paraformaldehyde; permeabilize with 0.1% Triton X-100 if intracellular epitope is targeted.
  • Block: 3% BSA or serum. Primary 1:200–1:800 in blocking buffer, 1 h RT or overnight 4°C. Use appropriate fluorescent secondary.

• Immunohistochemistry (general):

  • Retrieval: Heat-induced, citrate pH 6.0 or Tris-EDTA pH 9.0; titrate.
  • Primary: 1:100–1:500; detection via HRP/DAB or fluorescent methods.

• Flow cytometry (general):

  • 0.25–1 µg per 10^6 cells; 30 min on ice; wash with BSA-containing buffer.

Note: Dilutions and conditions are guidance only and are not validated for this specific item.

Biological Roles

Target overview (literature):

• Caveolin-3 (CAV3) is a muscle-specific component of caveolae—flask-shaped invaginations in the sarcolemma. It scaffolds signaling molecules, influences membrane mechanics, and participates in endocytosis and mechanotransduction.
• Expression: Predominantly in skeletal and cardiac muscle. It oligomerizes and interacts with caveolar lipids (cholesterol-rich domains) and partner proteins (e.g., dystrophin-associated complex components).
• Functional roles: Regulation of GPCR, EGFR, and nitric oxide signaling; involvement in T-tubule formation and maintenance; modulation of insulin signaling pathways in muscle.

Pathway and genetic context (literature):

• CAV3 gene encodes the muscle caveolin isoform; variants can disrupt caveolar structure and signaling.
• Protein–protein interactions: Caveolin scaffolding domain (CSD) binds consensus motifs in partner proteins; CAV3 can form hetero/oligomers with other caveolins under some conditions.

Use of an anti-Caveolin-3 antibody (general rationale):

• To probe CAV3 abundance, localization, and integrity in muscle cells and tissues.
• To distinguish caveolar vs. non-caveolar membrane domains via co-localization with markers such as Cavin proteins and dystrophin complex members.

Note: The above describes general biology of the target and does not constitute validation details for this specific Recombinant Caveolin-3 Antibody.

Buffer Applications

This section addresses buffers for antibody workflows; the antibody itself is not a buffering agent.

Item-specific buffer composition: Not specified for this item; refer to the product label/CoA.

General buffer guidance for anti-Caveolin-3 assays:

• Western blot: Tris–glycine SDS running buffer; transfer in Tris–glycine or CAPS (PVDF). Blocking in 5% milk or 3% BSA in TBS-T or PBS-T. Optimize antibody dilution in the same blocking buffer.
• Immunofluorescence/IHC: PBS or TBS for washes. Permeabilize with 0.1–0.3% Triton X-100 or saponin as needed; avoid harsh detergents that may disrupt caveolae excessively. Employ appropriate antigen retrieval for IHC (citrate pH 6.0 or Tris-EDTA pH 9.0), then titrate conditions.
• Flow cytometry: PBS/HEPES with 1–2% BSA and 0.05–0.1% sodium azide (if fixation). Maintain isotonicity to preserve cell integrity.
• Storage/working aliquots: PBS or HEPES at pH ~7.2–7.4 with stabilizers (e.g., carrier proteins, sugars) can improve stability; specifics for this item are not provided.

Caveats:

• Some buffers (e.g., high Tris/amine content) can interfere with amine-reactive labeling. Adjust buffers before conjugation.
• Validate buffer conditions empirically for your sample type and detection method.

Green Alternatives

Green chemistry substitution is not typically applicable to antibody reagents as they are supplied in aqueous buffers rather than organic solvents.

General sustainability considerations for antibody use:

• Minimize waste: Prepare working aliquots to reduce freeze–thaw cycles and product loss.
• Buffer selection: Use phosphate or HEPES buffers prepared from high-purity salts; avoid unnecessary organic additives.
• Preservatives: If sodium azide is present (not specified for this item), manage waste appropriately to prevent formation of heavy metal azides in plumbing. Consider preservative-free formulations only when sterility can be assured for cell-based assays.
• Energy use: Store at the lowest effective temperature recommended (−20°C for this item) and avoid ultra-low energy burdens unless required.

Comparison (general):

• Aqueous PBS vs. organic solvent systems: Aqueous systems have markedly lower environmental and safety burdens than organic solvent-based workflows typical of small-molecule chemistry.

Item-specific greener alternatives: Not applicable; no alternative formulations are specified for this item.

Pharmaceutical Uses

This product is designated for research use only; no medical or clinical applications are claimed.

General context (non-clinical):

• Antibodies against Caveolin-3 may be used in pharmaceutical research workflows, such as biomarker discovery, target validation, and mechanism-of-action studies in muscle biology.
• In process development contexts, research-grade antibodies can support assay development (e.g., ELISA capture/detection) and characterization of expression systems, but they are not qualified as GMP reagents or clinical diagnostics unless specifically stated.

Formulation/excipient notes (general):

• Research antibodies are commonly supplied in PBS or related buffers with optional preservatives (e.g., sodium azide) and stabilizers (e.g., BSA, sugars). For this item, formulation details are not specified; refer to the CoA/Spec Sheet.

Regulatory note:

• Not intended for use in humans or animals for therapeutic or diagnostic purposes. Any translational use would require additional qualification and regulatory compliance beyond the scope of this research reagent.

Physical Properties

Item-specific physical data: Not specified for this item; refer to CoA/Spec Sheet for buffer composition, concentration, and appearance.

General/literature values for antibodies (for context only):

• Typical molecular weight: ~150 kDa for full-length IgG; ~50 kDa for Fab’2 fragments; ~25–50 kDa for Fab/single-chain variants (literature). The exact format for this item is not specified.
• Isoelectric point (pI): Often ~6–9 depending on isotype/sequence (literature). Item-specific pI not provided.
• Solubility: Highly soluble in aqueous buffers (e.g., PBS, HEPES) with stabilizers (e.g., 0.02–0.05% sodium azide, 0.1–1% BSA or other carriers) (literature). Item-specific excipients not provided.
• Aggregation/colloidal behavior: Antibodies can self-associate depending on pH, ionic strength, and concentration; low-temperature storage and gentle handling help maintain monomeric state (literature).

Important cautions:

• Do not infer buffer composition, protein concentration, or stabilizers for this product—these are not specified for this item. Consult the product label or CoA.
• Viscosity and refractive index of aqueous antibody solutions closely match the underlying buffer; no item-specific values are provided.

Quality and Grades

Item-specific quality notes:

• Grade/Purity: Recombinant, ExactAb™, Validated (as provided). No additional purity specifications are listed for this item; refer to CoA/Spec Sheet for details.

What these terms generally imply (context, not a guarantee of methods for this item):

• Recombinant: Produced via controlled expression systems allowing sequence-defined antibodies and batch-to-batch consistency compared with hybridoma-derived materials.
• ExactAb™: Branding typically denotes sequence-defined, clonally consistent antibodies with standardized production. Specific platform attributes for this product are not detailed here.
• Validated: Indicates the antibody’s binding to Caveolin-3 has been demonstrated by one or more assays. However, the exact applications, species reactivity, and validation data for this item are not specified; consult the product documentation.

Analytical controls commonly associated with validated recombinant antibodies (general):

• Identity and purity by SDS-PAGE and LC–MS peptide map; monomer content by SEC-HPLC; endotoxin for cell-based compatibility; binding by ELISA/SPR/BLI; functional validation in immunoassays (WB/IHC/IF/FC). The specific tests performed for this item are not listed.

UV/LC specifications, stabilizers, and preservative content: Not specified for this item; refer to CoA/Spec Sheet.

Reaction and Applications

This product is an antibody reagent and is not a small-molecule reactant or catalyst. Synthetic organic reaction applications (e.g., cross-coupling, oxidations) do not apply.

Relevant application domains for antibodies (general, not item-validated):

• Immunoassays: Western blot (WB), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and flow cytometry (FC) to detect Caveolin-3.
• Quantitative binding: ELISA, BLI/SPR for affinity/kinetics to recombinant or native CAV3.
• Localization studies: Co-staining with membrane markers to map caveolae in muscle cells.

Manufacturer-validated applications for this specific item: Not specified; please consult the CoA/Spec Sheet. Any application should be optimized by the user, including antibody dilution, blocking, fixation, and antigen retrieval conditions.

Reaction Conditions

Classical chemical reaction conditions are not applicable. For immunoassay “binding reactions,” general conditions are provided for context (not item-specific validation):

• ELISA coating/binding (literature):
  • Antigen coating: 0.5–5 µg/mL in carbonate-bicarbonate buffer (pH 9.6), 2–16 h at 4°C or 1–2 h at 37°C.
  • Primary antibody incubation: Common starting range 0.05–1 µg/mL in blocking buffer, 1 h at RT or overnight at 4°C.
• Western blot (literature):
  • Primary antibody dilution: Often 1:500–1:5,000 in 3% BSA/TBST; incubate 1 h at RT or overnight at 4°C.
• Immunofluorescence/IHC (literature):
  • Primary incubation: 1–10 µg/mL or 1:100–1:1,000 in blocking buffer; 1 h at RT or overnight at 4°C.
• Flow cytometry (literature):
  • 0.1–5 µg/test depending on cell number (e.g., per 10^5–10^6 cells); 20–30 min at 4°C.

These are general starting points; optimize for the sample type, fixation/permeabilization, and detection system. Manufacturer-validated conditions for this specific item are not specified; consult CoA/Spec Sheet.

Safety and Handling

GHS classification (item-specific):

• Signal word, H-statements, pictograms: Not specified for this item; refer to the SDS for authoritative safety details.

General laboratory safety for antibody reagents (guidance only):

• PPE: Wear lab coat, safety glasses, and appropriate gloves. Avoid skin/eye contact and inhalation of aerosols.
• Handling: Work on ice or at 2–8°C during use to preserve activity. Avoid foaming and vigorous vortexing to limit aggregation.
• Incompatibilities: Avoid strong oxidizers and extreme pH that can denature proteins. If sodium azide is present (not specified), avoid contact with acids and copper/lead plumbing due to formation of heavy metal azides.
• Biological source: Recombinant protein reagents are non-sterile unless stated; handle as laboratory chemicals. No biological hazard claims are made for this product.

First aid (general):

• Skin/eye contact: Rinse with water for several minutes. Remove contaminated clothing. Seek medical attention if irritation persists.
• Ingestion/inhalation: Move to fresh air, rinse mouth, and seek medical attention as appropriate.

Spill and disposal:

• Wipe small spills with absorbent material, then clean with water and detergent. Dispose of waste according to institutional and local regulations. If azide-containing, segregate and flush drains with copious water (if permitted). Always defer to the SDS.

Solvent Selection

Not a small-molecule reagent; this is a recombinant antibody preparation typically supplied in an aqueous buffer.

Item-specific buffer/solvent details: Not specified for this item; refer to CoA/Spec Sheet and vial label.

General guidance (literature):

• Preferred media: Neutral aqueous buffers (e.g., PBS, 10–50 mM HEPES) at physiological ionic strength (100–300 mM NaCl) to maintain stability and binding.
• Additives: Stabilizers such as 0.02–0.05% sodium azide (biocide) and carrier proteins (e.g., 0.1–1% BSA or 0.1–0.5% trehalose) may be used to enhance stability; presence/levels are not specified for this item.
• Avoid: High proportions of organic solvents (acetonitrile, methanol, DMSO) that can denature antibodies. If antigen retrieval or permeabilization protocols use detergents/solvents, validate compatibility empirically.

Comparison (general):

• PBS (pH ~7.2–7.4): Broad compatibility with immunoassays; minimal buffering capacity under high protein loads.
• HEPES (pH stability across temperature): Useful for live-cell work (if preservative-free).
• Tris-buffered saline: Good protein solubility; beware of amine-reactive chemistries.

For experimental design, prioritize buffer systems recommended for the specific application (WB, IHC, IF, FC).

Storage and Reconstitution

Item-specific storage and shipping:

• Storage Conditions: Store at −20°C. Avoid repeated freezing and thawing (per Product Data).
• Shipped In: Ice chest + ice pads.

Item-specific formulation, concentration, and appearance: Not specified for this item; refer to the vial label and CoA/Spec Sheet.

General handling and reconstitution guidance (if lyophilized or for aliquoting; not item-specific):

• If lyophilized: Reconstitute with sterile, antibody-grade water or buffer (e.g., PBS) to the desired concentration; mix gently by inversion. Allow complete dissolution on ice. Avoid vigorous vortexing or foaming.
• If supplied in solution: Gently thaw on ice. Spin briefly to collect contents. Prepare single-use aliquots to minimize freeze–thaw cycles.
• Stabilizers: Addition of carrier protein (e.g., 0.1% BSA) or sugars (e.g., 5–10% trehalose) can improve stability for frozen stocks; ensure compatibility with downstream assays. Presence of stabilizers in this item is not specified.
• Short-term storage: 2–8°C for up to several days is common for working solutions; verify empirically. For long-term storage follow the stated −20°C guidance.
• Freeze–thaw: Avoid repeated cycles; aliquot immediately upon first thaw. Snap-freeze in dry-ice/ethanol or liquid nitrogen and store at −20°C (or lower if recommended in future documentation).

Always consult the product label and SDS for definitive instructions.

Structure and Identity

Item-specific facts (from Product Data):

• Product: Recombinant Caveolin-3 Antibody (SKU: Ab093262)
• Grade/Purity: Recombinant, ExactAb™, Validated
• Category: Antibody (research use only)
• CAS/CID/InChIKey/SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.

General/literature information (antibody biology context):

• Antibody type: Not specified for this item (e.g., IgG subclass, host species, recombinant format, Fc engineering not provided). Many recombinant research antibodies are human or rabbit IgG scaffolds; typical whole IgG is bivalent and heterotetrameric (2 heavy + 2 light chains).
• Target: Caveolin-3 (CAV3), a muscle-specific caveolin integral membrane protein associated with caveolae.
• 2D/structural description (antibody): Y-shaped immunoglobulin architecture comprising two identical heavy chains (~50 kDa each) and two identical light chains (~25 kDa each) linked by disulfide bonds; variable domains form the antigen-binding sites (paratopes). Exact sequence/engineered features for this item are not provided.

Notes on identity confirmation (general):

• Typical identity methods for recombinant antibodies include SDS-PAGE (reducing/non-reducing), intact mass by LC–MS, peptide mapping, and binding verification to antigen (ELISA, BLI/SPR). Specific methods used for this item are not specified.

Synthetic Utility

Not applicable to this product’s primary use. As a recombinant antibody, it is not a building block or reagent for small-molecule or polymer synthesis.

General notes for biochemical coupling (if users plan conjugation; literature):

• Antibody conjugation to enzymes, fluorophores, or nanoparticles typically uses amine-reactive (NHS esters), thiol-reactive (maleimides), or click-chemistry handles (azide–alkyne). Buffer exchange into amine-free buffers (e.g., PBS without primary amines) is recommended prior to NHS labeling.
• Maintain pH 7.2–8.5 for NHS ester coupling and pH ~6.5–7.0 for maleimide–thiol coupling. Desalt after reaction to remove unreacted label.

Item-specific reactive groups, degree-of-labeling constraints, or conjugation readiness are not specified for this antibody.

Target Specificity

Item-specific target designation:

• Target name: Caveolin-3 (CAV3)

Details not specified for this item (refer to CoA/Spec Sheet):

• Clone ID, recombinant sequence ID, or cataloged clone name: Not specified for this item.
• Host/recombinant scaffold and isotype/subclass (e.g., human IgG1, rabbit IgG): Not specified for this item.
• Epitope or immunogen region (e.g., N-terminus, CSD): Not specified for this item.
• Species reactivity (human/mouse/rat, etc.) and cross-reactivity: Not specified for this item.
• Verified applications (WB, IHC, IF, FC, IP) and positive control tissues/cell lines: Not specified for this item.

General considerations (literature):

• Caveolin-3 is a membrane-associated protein enriched in skeletal and cardiac muscle; selection of lysate/tissue controls typically includes human or rodent muscle. Epitope accessibility may depend on fixation/permeabilization and antigen retrieval conditions.

Conclusion: The antibody is indicated as recombinant and validated against Caveolin-3, but fine-grained specificity parameters must be obtained from the product’s CoA or validation data.

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Caveolin-3 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.