Recombinant DMAP1 Antibody - Primary antibody, specific to DMAP1, Rabbit IgG

Cat. No.: Ab100025

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human DMAP1 Antibody, Recombinant, could be used for WB, ICC/IF and so on.

Application

• WB: 1/500-1/1000
• ICC/IF: 1/50-1/200

Protein Function

Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.

Specifications

Product Name

Recombinant DMAP1 Antibody - Primary antibody, specific to DMAP1, Rabbit IgG

Sinónimos

DMAP 1 antibody | DMAP1 antibody | DMAP1_HUMAN antibody | DNA methyltransferase 1 associated protein 1 antibody | DNA methyltransferase 1-associated protein 1 antibody | DNMAP 1 antibody | DNMAP1 antibody | DNMT1 associated protein 1 antibody | DKFZp686L0

Especificações e pureza

ExactAb™, Validated, Recombinant, 0.5 mg/mL

Espécie hospedeira

Rabbit

Especificidade

DMAP1

Imunogénio

KLH-conjugated synthetic peptide encompassing a sequence within human DMAP1.

Controlo positivo

WB: HEK293, HeLa, THP-1, MCF7, Hep G2, Jurkat, NIH/3T3 and C6 cell lysates. ICC/IF: MCF7 cells.

Conjugação

Unconjugated

Grau

ExactAb™, Recombinant, Validated

Isótipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificação

Immunogen affinity purified

Concentração

0.5 mg/mL

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant DMAP1 Antibody (Ab100025)

Recombinant DMAP1 Antibody (Ab100025) - Western Blot
All lanes: Recombinant DMAP1 Antibody (Ab100025) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: 120.0 s

IF/ICC: Recombinant DMAP1 Antibody (Ab100025)

Recombinant DMAP1 Antibody (Ab100025) - IF/ICC
IImmunofluorescent analysis of Recombinant DMAP1 Antibody (Ab100025) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5 - 10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin -AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Aplicação

Aplicação

Dilution info

IF/ICC

1/50-1/200

WB

1/500-1/1000

Alvos associados (humanos)

DMAP1 Tbio DNA methyltransferase 1-associated protein 1 (0 Activities)

Discover Target: DMAP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant DMAP1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.