Recombinant DUSP6 Antibody - Primary antibody, specific to DUSP6, Rabbit IgG

Cat. No.: Ab100604

GRADE & PURITY Recombinant, Validated, ExactAb™, 1.25 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human DUSP6 Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/1000

IHC: 1/50-1/100

IF/ICC: 1/200

Protein Function

Inactivates MAP kinases. Has a specificity for the ERK family.

Specifications

Product Name

Recombinant DUSP6 Antibody - Primary antibody, specific to DUSP6, Rabbit IgG

Sinónimos

HH19 | MKP3 | PYST1 | DUSP6 | DUSP6a | Dual specificity phosphatase 6 | Dual specificity phosphatase 6 antibody | Dual specificity phosphatase 6 isoform a antibody | Dual specificity protein phosphatase 6 antibody | Dual specificity protein phosphatase PY

Especificações e pureza

ExactAb™, Validated, Recombinant, 1.25 mg/mL

Espécie hospedeira

Rabbit

Especificidade

DUSP6

Imunogénio

A synthetic peptide derived from human DUSP6 (AA 365-381).

Controlo positivo

WB: NIH/3T3 and C6 cell lysates; IHC: Human thyroid. IF/ICC: HeLa cells.

Conjugação

Unconjugated

Grau

ExactAb™, Recombinant, Validated

Isótipo

Rabbit IgG

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

1.25 mg/mL

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant DUSP6 Antibody (Ab100604)

Recombinant DUSP6 Antibody (Ab100604) - Western Blot
All lanes: Recombinant DUSP6 Antibody (Ab100604) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 38 kDa
Exposure time: 1.1s

IHC: Recombinant DUSP6 Antibody (Ab100604)

Recombinant DUSP6 Antibody (Ab100604) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid using Recombinant DUSP6 Antibody (Ab100604). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant DUSP6 Antibody (Ab100604)

Recombinant DUSP6 Antibody (Ab100604) - IF/ICC 

IF analysis of Recombinant DUSP6 Antibody (Ab100604) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant DUSP6 Antibody (Ab100604) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).

Aplicação

• Aplicação: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Alvos associados (humanos)

DUSP6 Tchem Dual specificity protein phosphatase 6 (0 Activities)

Discover Target: DUSP6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant DUSP6 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.