Recombinant SET7 Antibody - Primary antibody, specific to SET7, Rabbit IgG

Cat. No.: Ab127239

GRAU & PUREZA Recombinant, ExactAb™, Validated, 1.0 mg/mL

Application

WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human SET7 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function:
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.

Especificações

Product Name

Recombinant SET7 Antibody - Primary antibody, specific to SET7, Rabbit IgG

Sinónimos

FLJ21193 antibody | H3 K4 HMTase antibody | H3-K4-HMTase SETD7 antibody | H4 lysine 4 specific antibody | Histone H3 K4 methyltransferase antibody | Histone H3 lysine 4 specific methyltransferase antibody | Histone H3-K4 methyltransferase SETD7 antibody |

Especificações e pureza

ExactAb™, Validated, Recombinant, 1.0 mg/mL

Espécie hospedeira

Rabbit

Especificidade

SET7

Imunogénio

A synthetic peptide derived from human SETD7 (AA 1-30)

Controlo positivo

WB: Jurkat, HeLa, U-2 OS and C6 cell lysates.

Conjugação

Unconjugated

Grau

ExactAb™, Recombinant, Validated

Isótipo

Rabbit IgG

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

1.0 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant SET7 Antibody (Ab127239)

Recombinant SET7 Antibody (Ab127239) - Western Blot
All lanes: Recombinant SET7 Antibody (Ab127239) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 41 kDa
Observed band size: 44 kDa
Exposure time: 30.0 s

Aplicação

Aplicação

Dilution info

WB

1/500-1/1000

Alvos associados (humanos)

SETD7 Tchem Histone-lysine N-methyltransferase SETD7 (0 Activities)

Discover Target: SETD7

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant SET7 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.