Application
BIT-10 preservative is a colorless or pale yellow transparent liquid, which is an effective bacteriostatic agent for clinical blood cell analysis, biochemical analysis and immunoassay, including various reagents, quality control products, calibration products, diluents, cleaning solutions and buffers. Because the product has a wide range of the effect of inhibiting microbial growth, excellent compatibility and stability, low toxicity and biodegradability, BIT - 10 bacteriostatic agent as a kind of ideal, can replace sodium azide (drama) inflammable, explosive, thimerosal (including organic mercury) and gentamycin (yi resistance) traditional bacteriostatic agent such as a new type of antibacterial products. The product has a wide range of inhibition of microbial growth, good compatibility and stability, low toxicity and biodegradable, so that the diagnostic reagent has a longer period of validity and bottle opening validity. During the use of the clinical test instrument in the hospital, it is easy to catch bacteria.
Advantage
BIT-10 preservative has become an ideal and effective preservative for in vitro diagnostic reagents due to its broad spectrum activity, excellent compatibility and stability, and low toxicity at the application concentration. It is compatible with all kinds of emulsifiers, surfactants and other components, and can be dissolved in any alcohol or water. Strong stability, stored at -20℃ to 40℃ for a year without change, the active ingredient can withstand the high temperature of 150℃.Therefore, it has a long-term protection function, and can protect the system from the erosion of bacteria, mold and yeast. The fungicide is one of the new generation of industrial fungicides rising in the world. In the early 1980s imperial chemical industries invested 5.5 million pounds to develop a continuous process. The study on the synthesis of this fungicide was started in 1982 in China. The original powder of BIT is a solid powder with a melting point of 156℃, a solubility of 0.14% in water at 25℃, and a solubility of 1.5% at 90℃. However, the sodium salt or potassium salt of the product is easily soluble in water. The BIT-10 of crystal albicide is compounded with potassium hydroxide solution, which makes the product more stable. And PC isothiazolinone series of antibacterial agents to form a synergisticantibacterial effect. Its features and advantages include but are not limited to：
Broad-spectrum, long-acting kill a variety of bacteria, fungi and yeast, achieve good
bacteriostatic effect
is compatible with various emulsifiers, surfactants and other components, and can
be dissolved in alcohol and water
is stable, with no change at -20°C to 40°C for one year, and the active components
are stable at 150°C
is applicable to a wide range of pH, compatible with various enzymes, and does not
inhibit the binding of antibodies
is low toxicity and does not contain heavy metals or halogen compounds
is stable to amines
contains no formaldehyde, low odor and no VOC.
BIT-10 Preservative can replace Sodium Azide (NaN3) which widely applied to in vitro diagnostic reagents. In most cases (alkaline), Sodium Azide has a good antibacterial effect, but Sodium Azide can inhibit the activities of horseradish peroxidase (HRP), superoxide dismutase and other enzymes, so Sodium Azide cannot be added to the secondary antibody containing horseradish peroxidase. At the same time, Sodium Azide has the risks of explosion, very sensitive compounds with heavy metals and the risk of toxicity, inhibit cytochrome oxidase and other enzymes, and can block the formation of oxyhemoglobin in the body, leading to death. Due to the inflammable, explosive and highly toxic nature of Sodium Azide as a dangerous chemical, its procurement, storage, use and other links of the state have special regulatory requirements, its use is increasingly limited. BND-10 Preservative can also replace Thimerosal, which was widely applied to in vitro diagnostic reagent, it for both gram positive and negative of strong antibacterial ability, as the broad-spectrum antibacterial agent, because a small amount of organic mercury in theory has the nerve toxicity, so the use of thimerosal security issues by the attention of people.
Mechanism of Action
BIT-10 Preservative is a kind of broad spectrum antibacterial agent, the sterilization mechanism of BIT is the same as the assigned or different thiazole moiety ketone compounds of receptor cell membrane and cell wall with a strong penetration, after penetrate the cell periphery with cells containing sulphur proteins, enzymes, or simple molecular interaction, make its S -N key fracture, thus and receptor form S - S keys, destroy the normal function of cells. It is obvious that the weaker S - N bond of the isothiazolinone compound is beneficial to the antibacterial activity. But bit-10 is more stable than other antimicrobial agents, especially under alkaline conditions.
MIC
BIT-10 Preservative showed excellent anti-corrosion effect, its minimum inhibition concentration (MIC) as shown in the table below when the protein content of the reagents/solution is less than 10% of the formula. The product can be used for in vitro diagnostic reagents containing organic compounds, non-ionic or aniwonic surfactants and antigenic antibodies. The above components do not affect the antibacterial effect, nor do they produce antagonistic effects on each other. Compatible with most surfactants.
Storage
Store in cool place. Keep container tightly closed in a dry and well-ventilated place.
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