GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA
Especies hospedadoras
Mouse
Especificidad
Human Caspase-9
Inmunógeno
Recombinant protein corresponding to Human Caspase-9
Control positivo
WB: HeLa, HepG2, K562 and HEK293 whole cell lysates Flow Cytometry: K562 cells IF/ICC: HeLa cells
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Tipo de cadena ligera
kappa
SDS-PAGE
150.0 KDa
Método de purificación
Protein A purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Caspase-9 Mouse mAb (Ab183845) - Western Blot All lanes: Caspase-9 Mouse Antibody (Ab183845) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 46 kDa Observed band size: 52 kDa Exposure time: 10.0 s
Caspase-9 Mouse mAb (Ab183845) - Western Blot All lanes: Caspase-9 Mouse Antibody (Ab183845) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 46 kDa Observed band size: 53 kDa Exposure time: 15.0 s
Caspase-9 Mouse mAb (Ab183845) - Flow Cytometry Flow Cytometry analysis of K562 cells stained with Caspase-9 Mouse mAb (Ab183845) (Red) at 5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).
Caspase-9 Mouse mAb (Ab183845) - IF/ICC IF analysis of Caspase-9 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Caspase-9 Mouse mAb (Ab183845) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Aplicación
Aplicación
Dilution info
IF/ICC
1:500
Flow Cytometry
1/200 - 1/800
WB
1/1000 - 1/20000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
