CD11b Rat mAb (Biotin) - Primary antibody, specific to CD11b, Rat IgG2b

Cat. No.: Ab179521

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Human, Mouse

Isotype

Rat IgG2b

Clone Number

M1/70

Conjugation

Biotin

Descripción general

Application:
Flow: ≤0.1 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume.

Specifications

Product Name

CD11b Rat mAb (Biotin) - Primary antibody, specific to CD11b, Rat IgG2b

Sinónimos

antigen CD11b (p170) | CD11 antigen-like family member B | CD11b antigen | CD11b | Cell surface glycoprotein MAC-1 subunit alpha | Complement component 3 receptor 3 subunit | CR-3 alpha chain | CR3A | CR3AMGC117044 | Integrin alpha M | integrin alpha-M |

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Especies hospedadoras

Rat

Especificidad

CD11b

Inmunógeno

C57BL/10 splenocytes

Control positivo

Flow: Human peripheral blood monocyte

Conjugación

Biotin

Grado

ExactAb™, Validated

Isotipo

Rat IgG2b

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD11b Rat mAb (Biotin) (Ab179521)

CD11b Rat mAb (Biotin) (Ab179521) - Flow Cytometry
Flow cytometry analysis of CD11b expression by Human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD11b Rat mAb (Biotin) (Ab179521) at 0.01μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel – Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: CD11b Rat mAb (Biotin) (Ab179521)

CD11b Rat mAb (Biotin) (Ab179521) - Flow Cytometry
Flow cytometry analysis of CD11b expression by Human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with CD11b Rat mAb (Biotin) (Ab179521) at 0.01μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.1 μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

ITGAM Tbio Integrin alpha-M (0 Activities)

Discover Target: ITGAM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción