CD11b Mouse mAb - Primary antibody, specific to CD11b, Mouse IgG1

Cat. No.: Ab093725

GRADE & PURITY Carrier Free, Validated, Low Endotoxin, Azide Free, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Application

Flow Cytometry, IHC, IP, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

ICRF44

Conjugation

Unconjugated

Descripción general

Mouse anti Human CD11b Antibody, Monoclonal (ICRF44), could be used for Flow, WB, IHC, IP and so on.
Application
Flow: ≤ 1.0µg/100w cells  
WB: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
Protein Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Specifications

Product Name

CD11b Mouse mAb - Primary antibody, specific to CD11b, Mouse IgG1

Sinónimos

CD11B | antigen CD11b (p170) | CD11 antigen-like family member B | CD11b antigen | CD11b | Cell surface glycoprotein MAC-1 subunit alpha | Complement component 3 receptor 3 subunit | CR-3 alpha chain | CR3A | CR3AMGC117044 | Integrin alpha M | integrin al

Especificaciones y pureza

Carrier Free, Low Endotoxin, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Especies hospedadoras

Mouse

Especificidad

CD11b

Inmunógeno

Rheumatoid synovial cells and human monocytes.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, Low Endotoxin, PBS Only, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Flow Cytometry: CD11b Mouse mAb (Ab093725)

CD11b Mouse mAb (Ab093725) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD11b with CD11b Mouse mAb (Ab093725) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150). 

Flow Cytometry: CD11b Mouse mAb (Ab093725)

CD11b Mouse mAb (Ab093725) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD11b (red) with CD11b Mouse mAb (Ab093725) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• WB:

  Use at an assay dependent concentration.;

• IP:

  Use at an assay dependent concentration.

• IHC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  ≤ 1.0µg/100w cells ;

Objetivos asociados (humanos)

ITGAM Tbio Integrin alpha-M (0 Activities)

Discover Target: ITGAM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción