CD25 Rat mAb (AF488) - Primary antibody, specific to CD25

Cat. No.: Ab211656

CALIDAD & PUREZA ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

PC-61.5.3

Conjugation

AF488

Descripción general

Rat anti Mouse CD25 Antibody, Monoclonal (PC-61.5.3), could be used for Flow Cytometry

Especificaciones

Product Name

CD25 Rat mAb (AF488) - Primary antibody, specific to CD25

Sinónimos

Anti-mouse CD25 (IL-2Rα)-InVivo|In Vivo MAb anti-mouse CD25 (IL-2Rα)|CD25 Rat mAb|Interleukin 2 receptor alpha chain antibody|CD25 antibody|CD25 antigen antibody|IDDM10 antibody|IL 2 receptor alpha subunit antibody|IL-2 receptor subunit alpha antibody|IL-2-RA antibody|IL-2R subunit alpha antibody|IL2 RA antibody|IL2 Receptor alpha antibody|IL2-RA antibody|IL2R antibody|IL2R, alpha chain antibody|IL2RA antibody|IMD41 antibody|Interleukin 2 receptor alpha antibody|Interleukin 2 receptor antibody|Interleukin-2 receptor subunit alpha antibody|p55 antibody|t-cell growth factor receptor antibody|TAC antibody|TAC antigen antibody|TCGFR antibody

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Especies hospedadoras

Rat

Especificidad

CD25

Inmunógeno

IL-2-dependent cytolytic mouse T cell clone B6.1

Control positivo

Flow: C57BL/6 mouse splenocytes

Conjugación

AF488

Excitación (nm)

Blue Laser(488nm)

Emisión (nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

lambda

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD25 Rat mAb (AF488) (Ab211656)

CD25 Rat mAb (AF488) (Ab211656) - Flow Cytometry
Con A-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were stained with CD25 Rat mAb (AF488) (Ab211656) (red) compared to an isotype control (blue). Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Flow Cytometry: CD25 Rat mAb (AF488) (Ab211656)

CD25 Rat mAb (AF488) (Ab211656) - Flow Cytometry
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD25 Rat mAb (AF488) (Ab211656) (right panel) compared to an isotype control (left panel). Left panel - Rat IgG (AF488) (Ab190240).

Aplicación

Aplicación

Dilution info

Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Objetivos asociados (humanos)

IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)

Discover Target: IL2RA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)