CD25 Rat mAb (Biotin) - Primary antibody, specific to CD25, IgG1

Cat. No.: Ab179014
Disponible para pedir
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. 1.0 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG1
Clone Number
PC-61.5.3
Conjugation
Biotin
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μg
Ab179014-10μg
10
29,90US$
25μg
Ab179014-25μg
3
49,90US$
100μg
Ab179014-100μg
2
91,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Azide Free, 1.0 mg/mL Azide Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Specifications

Product Name
CD25 Rat mAb (Biotin) - Primary antibody, specific to CD25, IgG1
Sinónimos
Anti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25, Il2r, Ly-43 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2
Especificaciones y pureza
ExactAb™, Validated, Azide Free, 1.0 mg/mL
Especies hospedadoras
Rat
Especificidad
CD25
Inmunógeno
IL-2-dependent cytolytic mouse T cell clone B6.1
Control positivo
Flow: Murine splenocytes
Conjugación
Biotin
Grado
Azide Free, ExactAb™, Validated
Isotipo
IgG1
Tipo de cadena ligera
lambda
Método de purificación
Protein A High Salt
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Imágenes

CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry
BALB/c mouse splenocytes are stained with CD25 Rat mAb (Biotin) (Ab179014) and CD4 Rat mAb (APC) (Ab181112) (right), followed by Recombinant Streptavidin Protein (R-PE) (rp156150). BALB/c mouse splenocytes are stained with CD4 Rat mAb (APC) (Ab181112) and Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left).

CD25 Rat mAb (Biotin) (Ab179014) - Flow Cytometry
Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (Biotin) (Ab179014) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).

Aplicación
AplicaciónDilution info
Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
IL2RA Tclin Interleukin-2 receptor alpha chain (2 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mecanismos de acción
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeFechaArticulo
ZJ24F0404894Certificate of AnalysisJan 16, 2025 Ab179014
ZJ24F0404895Certificate of AnalysisJan 16, 2025 Ab179014
ZJ24F0404896Certificate of AnalysisApr 25, 2024 Ab179014
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide →

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