CD3 Rat mAb - Primary antibody, specific to CD3, Rat IgG2a

Cat. No.: Ab094429

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Activation, Flow Cytometry, Functional Assay, Stimulation

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

KT3

Conjugation

Unconjugated

Descripción general

Rat anti Mouse CD3 Antibody, Monoclonal (KT3), could be used for Flow, in vitro T cell negative selection, in vitro T cell stimulation/activation, ICC, IF and so on.

Application:
Flow: Use at an assay dependent concentration.

in vitro T cell negative selection: Use at an assay dependent concentration. 

in vitro T cell stimulation/activation: Use at an assay dependent concentration. 

ICC/IF: Use at an assay dependent concentration.

Protein Function
The CD3 complex mediates signal transduction.

Specifications

Product Name

CD3 Rat mAb - Primary antibody, specific to CD3, Rat IgG2a

Sinónimos

CD3 | T3e | CD3epsilon | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surfa

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Especies hospedadoras

Rat

Especificidad

CD3

Inmunógeno

CBAT6 mouse thymocytes

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotipo

Rat IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Imágenes

Flow Cytometry: CD3 Rat mAb (Ab094429)

CD3 Rat mAb (Ab094429) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD3 with CD3 Rat mAb (Ab094429) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD3 Rat mAb (Ab094429)

CD3 Rat mAb (Ab094429) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD3 (red) with CD3 Rat mAb (Ab094429) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• in vitro T cell negative selection:

  Use at an assay dependent concentration;

• in vitro T cell stimulation/activation:

  Use at an assay dependent concentration;

Objetivos asociados (humanos)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción