CD3 Rat mAb (PE) - Primary antibody, specific to CD3

Cat. No.: Ab185407

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

17A2

Conjugation

PE

Descripción general

Rat anti Mouse CD3 Antibody, Monoclonal (17A2), could be used for Flow Cytometry.

Specifications

Product Name

CD3 Rat mAb (PE) - Primary antibody, specific to CD3

Sinónimos

CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

The CD3 complex mediates signal transduction.

Especies hospedadoras

Rat

Especificidad

CD3

Inmunógeno

γδTCR-positive T-T hybridoma D1

Control positivo

Flow Cytometry: C57BL/6 Mouse splenocytes

Conjugación

PE

Excitación (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emisión (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD3 Rat mAb (PE) (Ab185407)

CD3 Rat mAb (PE) (Ab185407) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Rat mAb (PE) (Ab185407) at 1.0μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: CD3 Rat mAb (PE) (Ab185407)

CD3 Rat mAb (PE) (Ab185407) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD3 Rat mAb (PE) (Ab185407) at 1.0μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test; For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Objetivos asociados (humanos)

CD3G Tclin T-cell surface glycoprotein CD3 gamma chain (0 Activities)

Discover Target: CD3G

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)