Recombinant CD3 Antibody (AF405) - Primary antibody, specific to CD3

Cat. No.: Ab213110

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

P49A24

Conjugation

AF405

Descripción general

Recombinant CD3 Antibody, clone P49A24, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD3 Antibody (AF405) - Primary antibody, specific to CD3

Sinónimos

CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b

Especies hospedadoras

Rat

Especificidad

CD3

Inmunógeno

γδTCR-positive T-T hybridoma D1

Control positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugación

AF405

Excitación (nm)

Violet Laser(405nm)

Emisión (nm)

422nm

Ex/Em(nm)

Ex:401nm;Em:422nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: Recombinant CD3 Antibody (AF405) (Ab213110)

Recombinant CD3 Antibody (AF405) (Ab213110) - Flow Cytometry
Two-color flow cytometric analysis of CD3 expression on C57BL/6 Mouse splenocytes. C57BL/6 Mouse splenocytes cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (AF405) (Ab190070; Left panel) or Recombinant CD3 Antibody (AF405) (Ab213110; Right panel) at 0.1μg/test.

Flow Cytometry: Recombinant CD3 Antibody (AF405) (Ab213110)

Recombinant CD3 Antibody (AF405) (Ab213110) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD3 Antibody (AF405) (Ab213110) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Recombinant CD3 Antibody (AF405) (Ab213110)

Recombinant CD3 Antibody (AF405) (Ab213110) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD3 Antibody (AF405) (Ab213110) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

CD3G Tclin T-cell surface glycoprotein CD3 gamma chain (0 Activities)

Discover Target: CD3G

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)