CD44 Rat mAb (PE) - Primary antibody, specific to CD44

Cat. No.: Ab190175

CALIDAD & PUREZA ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

IM7

Conjugation

PE

Descripción general

Rat anti Mouse CD44 Antibody, Monoclonal (IM7), could be used for Flow Cytometry.

Especificaciones

Product Name

CD44 Rat mAb (PE) - Primary antibody, specific to CD44

Sinónimos

Ly-24 | Pgp-1 | HERMES | CD44 antigen | CD44 molecule (Indian blood group) | CD44 | CD44R | CDw44 | cell surface glycoprotein CD44 | chondroitin sulfate proteoglycan 8 | CSPG8 | ECMR-III | epican | Extracellular matrix receptor III | GP90 lymphocyte homin

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA

Especies hospedadoras

Rat

Especificidad

CD44

Inmunógeno

Dexamethasone-induced myeloid leukemia M1 cells

Control positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugación

PE

Excitación (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emisión (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD44 Rat mAb (PE) (Ab190175)

CD44 Rat mAb (PE) (Ab190175) - Flow Cytometry
Flow cytometry analysis of CD44 expression by mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD44 Rat mAb (PE) (Ab190175) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: CD44 Rat mAb (PE) (Ab190175)

CD44 Rat mAb (PE) (Ab190175) - Flow Cytometry
Flow cytometry analysis of CD44 expression by mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD44 Rat mAb (PE) (Ab190175) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Objetivos asociados (humanos)

CD44 Tbio CD44 antigen (0 Activities)

Discover Target: CD44

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD44 Rat mAb (PE)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.