CD45 Rat mAb (FITC) - Primary antibody, specific to CD45

Cat. No.: Ab185148
Disponible para pedir
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:498nm ? Fluorescence excitation maximum at 498 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:517nm ? Fluorescence emission maximum at 517 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2b
Clone Number
I3/2.3
Conjugation
FITC
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μg
Ab185148-10μg
3
37,90US$
25μg
Ab185148-25μg
2
75,90US$
100μg
Ab185148-100μg
1
235,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL Em:517nm,Ex:498nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Rat anti Mouse CD45 Antibody, Monoclonal (I3/2.3), could be used for Flow and so on.

Specifications

Product Name
CD45 Rat mAb (FITC) - Primary antibody, specific to CD45
Sinónimos
B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | CD45 Monoclonal Antibody (I3/2.3), FITC | FITC anti-mous
Especificaciones y pureza
ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL
Especies hospedadoras
Rat
Especificidad
CD45
Inmunógeno
Mouse lymphoma cell line
Control positivo
Flow: BALB/C mouse splenocytes.
Conjugación
FITC
Excitación (nm)
Blue Laser(488nm)
Emisión (nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
Grado
Em:517nm, Ex:498nm, ExactAb™, Validated
Método de purificación
Protein G purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Imágenes

CD45 Rat mAb (FITC) (Ab185148) - Flow Cytometry
Flow cytometry analysis of CD45 expression by BALB/C mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD45 Rat mAb (FITC) (Ab185148) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (FITC) (Ab180311).

CD45 Rat mAb (FITC) (Ab185148) - Flow Cytometry
Flow cytometry analysis of CD45 expression by BALB/C mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD45 Rat mAb (FITC) (Ab185148) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (FITC) (Ab180311). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación
AplicaciónDilution info
Flow Cytometry

≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mecanismos de acción
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeFechaArticulo
ZJ24F1012850Certificate of AnalysisMay 20, 2026 Ab185148
ZJ24F1012851Certificate of AnalysisMay 20, 2026 Ab185148
ZJ24F1012852Certificate of AnalysisMay 20, 2026 Ab185148
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:498nm grade guide → View Em:517nm grade guide →

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