Recombinant CD45.1 Antibody (Biotin) - Primary antibody, specific to Mouse CD45.1

Cat. No.: Ab213888

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG2a

Clone Number

P15B07

Conjugation

Biotin

Descripción general

Recombinant CD45.1 Antibody, clone P15B07, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD45.1 Antibody (Biotin) - Primary antibody, specific to Mouse CD45.1

Sinónimos

B220 antibody | CD45 antibody | CD45 antigen antibody | CD45R antibody | GP180 antibody | L-CA antibody | LCA antibody | LY5 antibody | Protein tyrosine phosphatase receptor type C antibody | Protein tyrosine phosphatase, receptor type, c polypeptide anti

Especificaciones y pureza

Validated, ExactAb™, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affec

Especies hospedadoras

Mouse

Especificidad

Mouse CD45.1

Inmunógeno

SJL mouse thymocytes and splenocytes.

Control positivo

Flow Cytometry: SJL mouse splenocytes.

Conjugación

Biotin

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: Recombinant CD45.1 Antibody (Biotin) (Ab213888)

Recombinant CD45.1 Antibody (Biotin) (Ab213888) - Flow Cytometry
Flow cytometry analysis of CD45.1 expression by SJL mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45.1 Antibody (Biotin) (Ab213888) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: Recombinant CD45.1 Antibody (Biotin) (Ab213888)

ecombinant CD45.1 Antibody (Biotin) (Ab213888) - Flow Cytometry
Flow cytometry analysis of CD45.1 expression by SJL mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45.1 Antibody (Biotin) (Ab213888) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test. For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)