CD86 Rat mAb - Primary antibody, specific to CD86, Rat IgG2a

Cat. No.: Ab095462

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, See COA

Application

Animal Model, Blocking, ELISA, Flow Cytometry, Functional Assay, IHC, IHC-F, IP, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

GL1

Conjugation

Unconjugated

Descripción general

 Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry, WB, IHC-F, IP, Blocking, ELISA, Functional Assay, Animal Model and so on.

Specifications

Product Name

CD86 Rat mAb - Primary antibody, specific to CD86, Rat IgG2a

Sinónimos

Activation B7-2 antigen | B70 | B7-2 antigen | B72 | B7-2 | B-lymphocyte activation antigen B7-2 | BU63 | CD28 antigen ligand 2 | CD28LG2B7-2 antigen) | CD86 antigen | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | FUN-1 | LAB72 | MGC34413 | T-lymp

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA

Mecanismos bioquímicos y fisiológicos

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d

Especies hospedadoras

Rat

Especificidad

CD86

Inmunógeno

LPS-activated CBA/Ca mouse splenic B cells

Control positivo

Flow: Mouse splenocytes.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotipo

Rat IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

Store at 2-8°C. Store at -20°C for long term. Upon delivery aliquot. Avoid freeze/thaw cycle.

Imágenes

Flow Cytometry: CD86 Rat mAb (Ab095462)

CD86 Rat mAb (Ab095462) - Flow Cytometry
Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 (red) with CD86 Rat mAb (Ab095462) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD86 Rat mAb (Ab095462)

CD86 Rat mAb (Ab095462) - Flow Cytometry
Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 with CD86 Rat mAb (Ab095462) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156150).

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  0.25μg/100w cells;

• WB:

  Use at an assay dependent concentration.;

• Blocking:

  Use at an assay dependent concentration.;

• Functional Assay:

  Use at an assay dependent concentration.;

• IP:

  Use at an assay dependent concentration.

• ELISA:

  Use at an assay dependent concentration.;

• Animal Model:

  Use at an assay dependent concentration. ;

• IHC-F:

  Use at an assay dependent concentration.

Objetivos asociados (humanos)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)

Discover Target: CD86

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD86 Rat mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.