Coelenterazine 400 a - ≥95% , CAS No.70217-82-2

CAS: 70217-82-2 Cat. No.: C332017 Peso molecular: 391.46

GRADE & PURITY ≥95%

Synonyms

Coelenterazine 400 a is known as a bisdeoxy derivative of coelenterazine. | BlueSyn C | 2,8-dibenzyl-6-phenylimidazo[1,2-a]pyrazin-3-ol | coelenterazine-400a | 2,8-dibenzyl-6-phenyl-7H-imidazo[1,2-a]pyrazin-3-one | AKOS030213259 | C-7010 | DTXSID10628592

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Descripción general

product description：

Coelenterazine 400 a is a chromophore that produces the chemiluminescence displayed by the Aequorin protein in the presence of Ca|2+|. Aequorin is employed as a chemiluminescent probe for intracellular Ca|2+|, and derivatives of this protein generated with various Coelenterazine derivatives produced a range of Ca|2+|probes with varying spectral properties. Coelenterazine 400 a is a bisdeoxy derivative of coelenterazine. Coelenterazine 400 a displays an emission maximum of 395nm following conversion by Renilla luciferase (Rluc). Coelenterazine 400 a is commonly paired with class 1 and 3 GFP acceptors.

Applications：

Coelenterazine 400 a is a blue emitting Rluc substrate for bioluminescence resonance energy transfer 2 (BRET2) studies.

Specifications

Sinónimos

Coelenterazine 400 a is known as a bisdeoxy derivative of coelenterazine. | BlueSyn C | 2, 8-dibenzyl-6-phenylimidazo[1, 2-a]pyrazin-3-ol | coelenterazine-400a | 2, 8-dibenzyl-6-phenyl-7H-imidazo[1, 2-a]pyrazin-3-one | AKOS030213259 | C-7010 | DTXSID10628592

Especificaciones y pureza

≥95%

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -20°C, Argon charged

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Nota

Stock Solution1. Let the vial warm to room temperature before opening to prevent condensation from entering the product.2. Dissolve the product in ethanol at a concentration of 0.4 mg/ml. Mix gently.3. Avoid solvents such as DMSO as they may cause oxidati

Pureza

≥95%

Nombres e identificadores

• Pubchem Sid: 504773439
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504773439
• Sonrisas canónicas: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• IUPAC Name: 2,8-dibenzyl-6-phenylimidazo[1,2-a]pyrazin-3-ol
• InChIKey: XNNYOKUWNAAJQW-UHFFFAOYSA-N
• INCHI: 1S/C26H21N3O/c30-26-23(17-20-12-6-2-7-13-20)28-25-22(16-19-10-4-1-5-11-19)27-24(18-29(25)26)21-14-8-3-9-15-21/h1-15,18,30H,16-17H2
• Isómeros SMILES: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• Peso molecular: 391.46
• Reaxy-Rn: 36019358
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=36019358&ln=

Propiedades químicas y físicas

• Solubilidad: Soluble in 0.1% warm acetone. Hardly soluble in water.
• Índice de refracción: n20D1.66 (Predicted)
• Punto de ebullición (°C): 551.39° C at 760 mmHg (Predicted)
• Punto de fusión (°C): 94-98° C
• Peso molecular: 391.500 g/mol
• XLogP3: 6.200
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 5
• Exact Mass: 391.168 Da
• Monoisotopic Mass: 391.168 Da
• Topological Polar Surface Area: 50.400 Ų
• Heavy Atom Count: 30
• Formal Charge: 0
• Complexity: 524.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Referencias

1. Liu Wang, Yangyi Liu, Jing Sun, Juanjuan Su, Jing Feng, Li Miao, Weijie Zhao, Hongjie Zhang, Kai Liu.  (2025)  In Situ Ultrasound-Triggered Bioluminescence for Combined Sono/Photodynamic Immunotherapy.  ACS Nano,      [PMID:40671351] [10.1021/acsnano.5c06999]