DAF-FM DA - ≥98%(HPLC) , CAS No.254109-22-3

CAS: 254109-22-3 Cat. No.: D131469 Peso molecular: 496.42

GRADE & PURITY ≥98%(HPLC)

Synonyms

3-Amino, 4-aminomethyl-2',7'-difluorofluorescein Diacetate | MolMap_000015 | MFCD16872050 | DAF-FM DA | J-100010 | FT-0624398 | AKOS027380925 | DAF-FM DA, >=98% (HPLC) | DAF-FM DA (cell permeable) | Daf-fm da(cell permeable) | [6'-acetyloxy-4-amino-2',7'-

Storage

Protegido de la luz,Almacenar a -20°C

Shipped In

Hielera + almohadillas de hielo

Descripción general

El diacetato DAF-FM es un reactivo importante para la cuantificación de bajas concentraciones de óxido nítrico en solución. Este compuesto es esencialmente no fluorescente hasta que reacciona con el NO para formar un benzotriazol fluorescente. Con máximos de excitación/emisión de 495

Specifications

Sinónimos

3-Amino, 4-aminomethyl-2', 7'-difluorofluorescein Diacetate | MolMap_000015 | MFCD16872050 | DAF-FM DA | J-100010 | FT-0624398 | AKOS027380925 | DAF-FM DA, >=98% (HPLC) | DAF-FM DA (cell permeable) | Daf-fm da(cell permeable) | [6'-acetyloxy-4-amino-2', 7'-

Especificaciones y pureza

≥98%(HPLC)

Mecanismos bioquímicos y fisiológicos

DAF-FM DA es una sonda fluorescente permeable a las células para la detección de óxido nítrico (NO). DAF-2 es el compuesto original y se utiliza ampliamente, pero DAF-FM y DAF-FM DA son más sensibles al óxido nítrico (NO) en comparación con DAF-2 y más fo

Condiciones de almacenamiento de almacenamiento

Protegido de la luz, Almacenar a -20°C

Enviado en

Hielera + almohadillas de hielo

Nota

Siempre que sea posible, prepare y utilice las soluciones el mismo día. Sin embargo, si necesita preparar soluciones madre con antelación, le recomendamos que almacene la solución como alícuotas en viales herméticamente cerrados a -20°C. Por lo general, podrán utilizarse durante un mes. Antes de su uso, y antes de abrir el vial, le recomendamos que deje que el producto se equilibre a temperatura ambiente durante al menos 1 hora. ¿Necesita más consejos sobre solubilidad, uso y manipulación? Visite nuestra página de preguntas frecuentes (FAQ) para obtener más información.

Pureza

≥98%(HPLC)

Nombres e identificadores

• Sonrisas canónicas: CC(=O)OC1=C(C=C2C(=C1)OC3=CC(=C(C=C3C24C5=C(C(=C(C=C5)NC)N)C(=O)O4)F)OC(=O)C)F
• IUPAC Name: [6'-acetyloxy-4-amino-2',7'-difluoro-5-(methylamino)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] acetate
• InChIKey: BEVHTVRRVVEMEF-UHFFFAOYSA-N
• INCHI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• Isómeros SMILES: CC(=O)OC1=C(C=C2C(=C1)OC3=CC(=C(C=C3C24C5=C(C(=C(C=C5)NC)N)C(=O)O4)F)OC(=O)C)F
• WGK Alemania: 3
• Peso molecular: 496.42
• Reaxy-Rn: 28981309
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=28981309&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Clase Benzopyrans
      • Subclass 1-benzopyrans
        • Level5 Dibenzopyrans
          • Level6 Xanthenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Clase: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Dibenzopyrans
• Direct Parent: Xanthenes
• Alternative Parents: Diarylethers Phthalides Benzofuranones Tricarboxylic acids and derivatives Isobenzofurans Secondary alkylarylamines Aryl fluorides Benzenoids Vinylogous amides Amino acids and derivatives Carboxylic acid esters Lactones Oxacyclic compounds Organic oxides Carbonyl compounds Organofluorides Organopnictogen compounds Primary amines Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Xanthene - Diaryl ether - Benzofuranone - Isobenzofuranone - Phthalide - Isocoumaran - Isobenzofuran - Tricarboxylic acid or derivatives - Secondary aliphatic/aromatic amine - Aryl fluoride - Aryl halide - Benzenoid - Vinylogous amide - Amino acid or derivatives - Carboxylic acid ester - Lactone - Secondary amine - Oxacycle - Carboxylic acid derivative - Ether - Hydrocarbon derivative - Organohalogen compound - Organofluoride - Organic oxide - Amine - Organonitrogen compound - Carbonyl group - Organopnictogen compound - Organic oxygen compound - Organic nitrogen compound - Primary amine - Organooxygen compound - Aromatic heteropolycyclic compound
• Descripción: This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Propiedades químicas y físicas

• Sensibilidad: Hygroscopic；Light sensitive
• Peso molecular: 496.400 g/mol
• XLogP3: 3.700
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 11
• Rotatable Bond Count: 5
• Exact Mass: 496.108 Da
• Monoisotopic Mass: 496.108 Da
• Topological Polar Surface Area: 126.000 Ų
• Heavy Atom Count: 36
• Formal Charge: 0
• Complexity: 862.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Referencias

1. Jie He, Pan Ran, Maohua Chen, Wenxiong Cao, Shuang Xie, Guiyuan Zhang, Xiaohong Li.  (2022)  Intracellular infection-responsive release of NO and peptides for synergistic bacterial eradication.  JOURNAL OF CONTROLLED RELEASE,      [PMID:36243236] [10.1016/j.jconrel.2022.10.015]

2. Yang Zheng, Jiaru Wang, Tiejian Zhao, Lei Wang, Jiahui Wang.  (2021)  Modulation of the VEGF/AKT/eNOS signaling pathway to regulate liver angiogenesis to explore the anti-hepatic fibrosis mechanism of curcumol.  JOURNAL OF ETHNOPHARMACOLOGY,      [PMID:34358654] [10.1016/j.jep.2021.114480]

3. Qi Li, Shuyuan Yu, Yuxuan Wang, Hui Zhao, Ziqi Gao, Huilong Du, Huayong Yang, Luqi Shen, Hongzhao Zhou.  (2025)  Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions.  TRENDS IN BIOTECHNOLOGY,      [PMID:39779422] [10.1016/j.tibtech.2024.11.019]

4. Sixin Zhang, Han Lv, Bin Cui, Dandan Zhou.  (2025)  NO-driven Anammox systems contribute to nitrogen removal via altering nitrogen transformation pathways and microbial interactions.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.167030]