E235 - ≥98% , CAS No.891894-69-2

CAS: 891894-69-2 Cat. No.: E651168 Peso molecular: 484.59

GRADE & PURITY ≥98%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

E235 is an expression regulator of activates transcription factor 4 ( ATF4 ). E235 reduces cell viability by activating integrated stress response (ISR) and DNA damage response signals. E235 has anti-proliferative activity and can be used for tumor research

In Vitro

E235 (1 µM; 2, 4, 8, 16 or 24 h) down-regulates the level of XBP-1s mRNA in HT1080 cells with time dependent manner. E235 (0.1-10 µM; 4 d or 5 d) has anti-proliferative activity on HT1080, RPMI-8226, B16F10, 4T1, HT1080 shNT and HT1080 shATF4 cells. E235 (0, 1, 5 and 10 µM; 4 h) increases the expression of p53 with dose-dependent manner in AG1522 cells. E235 (1 µM; 2 h) increases the expression of p-Chk2 in AG1522 cells. E235 (0, 1, 5 and 10 µM; 2 h) increases the expression of p-p53 in AG1522 and HT1080 cells. E235 (0, 0.5, 1, 5 and 10 µM; 0.5 h) increases the expression of γ-H2AX with dose-dependent manner in HT1080 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: HT1080, B16F10 and AG1522 cells. Concentration: 0, 1, 5 and 10 µM. Incubation Time: 4 h. Result: Increased the expression of ATF4 with dose-dependent manner. Western Blot AnalysisCell Line: HT1080 cells. Concentration: 0, 0.5, 1, 5 or 10 µM. Incubation Time: 2, 4 or 8 h. Result: Increased the expression of p-elF2α with time and dose dependent manner. Cell Proliferation AssayCell Line: HT1080 or RPMI-8226 cells. Concentration: 1 µM. Incubation Time: 0, 8, 16 and 24 h. Result: Exhibited anti-proliferative activity. Cell Viability AssayCell Line: AG1522 cells. Concentration: 0, 0.1, 0.5, 1 and 10 µM. Incubation Time: 4 d. Result: Inhibited cell viability with dose-dependent manner. Cell Cycle AnalysisCell Line: HT1080 cells and B16F10 cells. Concentration: 1 µM. Incubation Time: 8, 16 and 24 h. Result: Caused cell arrest during G2/M phase.

Form:Solid

IC50& Target:Activates transcription factor 4, ATF4

Specifications

Especificaciones y pureza

≥98%

Mecanismos bioquímicos y fisiológicos

E235 is an expression regulator of activates transcription factor 4 ( ATF4 ). E235 reduces cell viability by activating integrated stress response (ISR) and DNA damage response signals. E235 has anti-proliferative activity and can be used for tumor resear

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

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Tipo de acción

ACTIVATOR

Pureza

≥98%

Nombres e identificadores

• Peso molecular: 484.59