ERCC1-XPF-IN-2 - ≥98% , CAS No.1808986-37-9

CAS: 1808986-37-9 Cat. No.: E650443 Peso molecular: 326.17

GRADE & PURITY ≥98%

Storage

Protected from light,Store at -80°C

Shipped In

Dry ice packs + Cold packs

Descripción general

ERCC1-XPF-IN-2 is a potent ERCC1-XPF endonuclease inhibitor with an IC 50 value of 0.6 µM. ERCC1-XPF-IN-2 shows activity in nucleotide excision repair, cisplatin enhancement and γH2AX assays

In Vitro

ERCC1-XPF-IN-2 (compound 13) (0-100 µM) shows FEN-1 and DNase I activity with IC 50 s of >100, >100 µM, respectively. ERCC1-XPF-IN-2 slow binding kinetics with an K d value of ~30 µM. ERCC1-XPF-IN-2 shows not toxic to Hep-G2 cells at 10 µM and relatively short mouse and human microsomal half-lives with t 1/2 value of 23 min and 28 min for mouse and human, respectively.\nERCC1-XPF-IN-2 (0-60 µM; 24 h) shows inhibition of nucleotide excision repair (NER) with an IC 50 value of 15.6 μM in A375 cells. ERCC1-XPF-IN-2 (0-60 µM) increases the cisplatin activity with no toxicity. ERCC1-XPF-IN-2 (10 µM; 6h) causes a delay in DNA repair by a right shift towards higher numbers of γH2AX foci per cell. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cytotoxicity AssayCell Line: A375 cells Concentration: 0-60 µM Incubation Time: Result: Showed no toxicity and increased the cisplatin activity up to 1.5-fold (PF50).

Form:Solid

IC50& Target:ERCC1-XPF

Specifications

Especificaciones y pureza

≥98%

Mecanismos bioquímicos y fisiológicos

ERCC1-XPF-IN-2 is a potent ERCC1-XPF endonuclease inhibitor with an IC 50 value of 0.6 µM. ERCC1-XPF-IN-2 shows activity in nucleotide excision repair, cisplatin enhancement and γH2AX assays.

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -80°C

Enviado en

Dry ice packs + Cold packs

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Tipo de acción

INHIBITOR

Pureza

≥98%

Nombres e identificadores

• Peso molecular: 326.17

Propiedades químicas y físicas

• Solubilidad: DMSO : ≥ 250 mg/mL (766.47 mM)