Suspend 41g of the medium in 950ml of purified water. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Autoclave for 15 minutes at 121ºC (15psi). Cool to 45-50°C. Aseptically add 50ml of a sterile egg yolk suspension and 2ml of a filter sterilized aqueous solution of polymyxin B.
Component
g/liter
Enzymatic Digest of Casein
1
Mannitol
10
Sodium Chloride
2
Magnesium Sulfate
0.1
Disodium Phosphate
2.5
Monopotassium Phosphate
0.25
Bromthymol Blue
0.1
Sodium Pyruvate
10
Agar
15
Total: 40.95g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
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