DEFB135 Mouse mAb: Applications, Specificity & Usage Guide

DEFB135 Mouse mAb is a monoclonal antibody raised in mouse that targets the human beta-defensin 135 protein, offering researchers a reliable tool for investigating innate immune defense mechanisms in relevant immunoassay applications.

Product SKU

Ab350340

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

DEFB135 (Beta-Defensin 135)

Formulation / Buffer

PBS only

Host Species

Mouse

External Reference

absea #KC-48653

Target Overview: What Is DEFB135?

DEFB135, also known as Beta-Defensin 135, is a member of the beta-defensin family of small cationic antimicrobial peptides. Beta-defensins are key effectors of the innate immune system, expressed primarily at epithelial surfaces where they provide a first line of defense against bacteria, fungi, and viruses. DEFB135 is encoded by a gene within the large beta-defensin gene cluster on human chromosome 8p23, a region notable for copy-number variation that has been linked to susceptibility to infectious and inflammatory diseases.

While the precise biological functions of DEFB135 remain an active area of research, beta-defensins in general are known to exhibit direct antimicrobial activity, recruit immune cells via chemokine receptor interactions, and participate in linking innate and adaptive immunity. Investigating the expression and regulation of DEFB135 may therefore yield important insights into host defense at mucosal surfaces.

Antibody Characteristics & Formulation

DEFB135 Mouse mAb (Aladdin SKU: Ab350340; absea catalog reference KC-48653) is a monoclonal antibody generated in mouse hosts. Monoclonal antibodies provide a single, homogeneous population of immunoglobulin molecules recognizing one specific epitope on the target antigen, ensuring high reproducibility and lot-to-lot consistency across experiments.

The antibody is supplied in PBS (Phosphate-Buffered Saline) only, without additional stabilizers such as BSA or glycerol. Researchers should take note of this lean formulation when planning long-term storage or dilution series: it is generally advisable to add a carrier protein (e.g., 0.1% BSA) and a cryoprotectant (e.g., 50% glycerol) before aliquoting for storage at -20 °C to minimize freeze-thaw-induced aggregation and activity loss.

Note: Full details such as isotype, clone identity, exact immunogen sequence, and validated applications are not included in the current source material. Please consult the official Aladdin product datasheet or contact technical support for complete specifications.

Recommended Applications & Experimental Considerations

Mouse monoclonal antibodies targeting beta-defensin family members are commonly employed in a range of standard immunological techniques. Depending on validated performance data (to be confirmed on the official datasheet), DEFB135 Mouse mAb may be suitable for applications including:

• Western Blot (WB): Detection of DEFB135 in denatured protein lysates from relevant tissue or cell line samples.
• Enzyme-Linked Immunosorbent Assay (ELISA): Quantification of DEFB135 in supernatants or biological fluids, either as a capture or detection antibody in sandwich formats.
• Immunofluorescence (IF) / Immunohistochemistry (IHC): Localization of DEFB135 expression within fixed cells or tissue sections.
• Immunoprecipitation (IP): Enrichment of native DEFB135 protein for downstream interaction or activity studies.

Always optimize primary antibody concentrations, blocking conditions, and secondary antibody pairings empirically for each application. Because the formulation is PBS-only, prepare fresh working dilutions in an appropriate diluent buffer supplemented with carrier protein to maintain antibody stability during incubation steps.

Storage, Handling & Quality Assurance

Storage: Upon receipt, briefly centrifuge the vial to collect contents at the bottom. For short-term use (up to 1 month), store at 4 °C. For long-term storage, supplement with a cryoprotectant, aliquot into single-use volumes, and store at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles, which can lead to antibody aggregation and reduced immunoreactivity.

Handling: Maintain the antibody on ice during experimental setup. Use sterile, low-protein-binding tubes and pipette tips to prevent nonspecific adsorption. Because the PBS-only buffer lacks preservatives, maintain aseptic technique to prevent microbial contamination.

Quality Assurance: As with all Aladdin research reagents, each lot of DEFB135 Mouse mAb undergoes quality-control testing prior to release. Researchers are encouraged to run a positive control (a cell line or tissue known to express DEFB135) alongside experimental samples to verify antibody performance in their specific system.

Frequently asked questions

What buffer is DEFB135 Mouse mAb (Ab350340) supplied in?

DEFB135 Mouse mAb is supplied in PBS (Phosphate-Buffered Saline) only, without added stabilizers such as BSA or glycerol. Users planning long-term storage should supplement with appropriate cryoprotectants before aliquoting.

What species and cell types are expected to express DEFB135?

DEFB135 is a human beta-defensin family member primarily associated with expression at epithelial surfaces, including respiratory, urogenital, and skin epithelia, consistent with the known distribution of beta-defensin genes. Specific validated expression data for this antibody should be confirmed in the official product datasheet.

How should I prevent performance loss during freeze-thaw cycles?

Aliquot the antibody into single-experiment volumes immediately after receipt to avoid repeated freeze-thaw cycles. Adding 50% glycerol as a cryoprotectant and 0.1% BSA as a carrier protein before freezing at -20 °C or -80 °C will help maintain antibody integrity and immunoreactivity over time.

Which secondary antibodies are compatible with DEFB135 Mouse mAb?

Because DEFB135 Mouse mAb is generated in mouse, anti-mouse secondary antibodies conjugated to HRP, fluorescent dyes, or biotin are appropriate choices depending on your detection method. Always verify that the secondary antibody is validated for your specific application (WB, IHC, IF, etc.) and titrate it empirically to minimize background.

References

1. Harder J, Schroder JM, Glaser R. The skin surface as antimicrobial barrier: present concepts and future outlooks. Exp Dermatol. 2013;22(1):1-5.
2. Hollox EJ, Huffmeier U, Zeeuwen PL, et al. Psoriasis is associated with increased beta-defensin genomic copy number. Nat Genet. 2008;40(1):23-25.
3. Aladdin Scientific. Product Datasheet: DEFB135 Mouse mAb, SKU Ab350340. Aladdin Scientific; accessed 2024.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.