QDPR Mouse mAb: Selection, Application & Protocol Guide

QDPR Mouse mAb is a monoclonal antibody raised in mouse, designed to specifically detect quinoid dihydropteridine reductase (QDPR) in research applications including immunoassays and protein-expression studies.

SKU

Ab350667

Antibody Type

Mouse Monoclonal Antibody (mAb)

Formulation

PBS Only (no carrier protein, no azide)

Target Protein

QDPR (Quinoid Dihydropteridine Reductase)

Host Species

Mouse

Reference Code

absea#KC-46464

Introduction to QDPR and Its Biological Significance

Quinoid dihydropteridine reductase (QDPR) is an enzyme that plays a critical role in the regeneration of tetrahydrobiopterin (BH4), an essential cofactor for aromatic amino acid hydroxylases including phenylalanine hydroxylase, tyrosine hydroxylase, and tryptophan hydroxylase. These enzymes are indispensable for the biosynthesis of neurotransmitters such as dopamine, serotonin, and norepinephrine.

Deficiency in QDPR is associated with hyperphenylalaninemia and atypical phenylketonuria (PKU), making QDPR a clinically relevant research target. Accurate detection of QDPR protein levels is therefore important in metabolic disease research, neuroscience, and enzyme biology studies.

Product Overview and Formulation Details

The QDPR Mouse mAb (SKU: Ab350667) is a monoclonal antibody produced in mouse hosts, offering high specificity and batch-to-batch consistency compared to polyclonal alternatives. The antibody is supplied in PBS-only formulation, meaning it contains no carrier proteins (such as BSA) and no preservatives such as sodium azide.

This PBS-only format is particularly advantageous for applications requiring direct conjugation, such as labeling with fluorophores, enzymes, or biotin, as carrier proteins can interfere with conjugation chemistry. Researchers performing conjugation or sensitive downstream assays should note this formulation characteristic when designing experiments.

Key product identifiers:

• SKU: Ab350667
• Reference code: absea#KC-46464
• Host: Mouse
• Format: PBS Only

Recommended Storage and Handling Guidelines

Proper storage and handling are essential to maintaining the activity and specificity of QDPR Mouse mAb over time. Follow these general best-practice guidelines:

• Short-term storage (up to 1 month): Store at 4°C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot the antibody upon receipt and store at -20°C or -80°C. Single-use aliquots help prevent degradation from repeated thawing.
• Avoid: Prolonged exposure to room temperature, direct light, and repeated freeze-thaw cycles, all of which can denature the antibody and reduce its binding efficacy.
• PBS-only formulation note: Because this product lacks BSA or glycerol stabilizers, extra care should be taken to minimize freeze-thaw cycles. Consider adding a carrier protein (e.g., 0.1% BSA) if long-term stability at 4°C is required, unless downstream conjugation is planned.

Always centrifuge briefly before opening to recover any material from the cap or sides of the tube.

Application and Dilution Protocol

QDPR Mouse mAb is intended for use in standard immunological research applications. While specific validated dilutions for this lot should be confirmed in the product datasheet, the following general starting-point guidelines apply for monoclonal antibodies of this class:

• Western Blot (WB): Typically 1:500–1:2000. Dilute in blocking buffer (e.g., 5% non-fat milk or BSA in TBST). Incubate membrane with primary antibody overnight at 4°C.
• Immunofluorescence (IF): Typically 1:50–1:200. Fix and permeabilize cells prior to incubation. Use appropriate secondary anti-mouse antibody conjugated to a fluorophore.
• Immunohistochemistry (IHC): Typically 1:50–1:200. Antigen retrieval may be required depending on fixation method.
• ELISA: Titration recommended; start at 1:1000 and optimize empirically.

Always perform a titration experiment to determine the optimal working concentration for your specific experimental system and sample type. Use an appropriate isotype control (mouse IgG) run in parallel to assess non-specific background.

Note: Specific validated application data for Ab350667 should be obtained from the official product datasheet. Contact Aladdin Scientific technical support for application-specific guidance.

Troubleshooting Common Issues

If unexpected results occur when using QDPR Mouse mAb, consult the following troubleshooting guide:

• No signal detected: Verify that the antibody has been stored correctly and has not been subjected to excessive freeze-thaw cycles. Confirm that the target protein is expressed in your cell or tissue model. Check secondary antibody compatibility (must be anti-mouse).
• High background: Increase blocking time or concentration. Optimize primary antibody dilution. Ensure wash steps are thorough (3×10 min in TBST). If performing IHC, verify that endogenous peroxidase has been quenched.
• Multiple non-specific bands (WB): Reduce primary antibody concentration. Increase stringency of wash buffer (add 0.05–0.1% Tween-20). Confirm sample preparation did not degrade the protein.
• Weak signal: Increase antibody concentration or extend incubation time. Check that detection system sensitivity is adequate. Ensure sufficient protein was loaded (for WB) or antigen is accessible (for IHC/IF).

For issues not resolved by the above steps, contact Aladdin Scientific technical support with your experimental details, lot number, and images where applicable.

Frequently asked questions

What applications is QDPR Mouse mAb (Ab350667) validated for?

The QDPR Mouse mAb is intended for standard immunological research applications such as Western blot, immunofluorescence, immunohistochemistry, and ELISA. Researchers should consult the official product datasheet for specifically validated applications and recommended dilutions for Ab350667.

Why is the QDPR Mouse mAb supplied in PBS-only format, and does this affect storage?

The PBS-only formulation means the antibody contains no BSA carrier protein or azide preservative, making it ideal for direct conjugation workflows. However, this also means the antibody may be slightly less stable during long-term storage at 4°C. It is recommended to aliquot upon receipt and store at -20°C or -80°C to minimize freeze-thaw cycles and preserve activity.

What secondary antibody should be used with QDPR Mouse mAb?

Since QDPR Mouse mAb is raised in mouse, you must use an anti-mouse secondary antibody for detection. Choose a secondary antibody conjugated to the appropriate reporter (HRP for WB/IHC, fluorophore for IF) and ensure it is validated for your experimental species and application to avoid cross-reactivity.

How should I determine the optimal working dilution for my experiment?

Begin with the dilution ranges recommended in the product datasheet or this guideline, then perform a titration experiment across a range of concentrations to identify the dilution that gives the strongest specific signal with the lowest background in your specific system. Always include an isotype control for comparison.

What is QDPR and why is it an important research target?

QDPR (Quinoid Dihydropteridine Reductase) is an enzyme essential for regenerating tetrahydrobiopterin (BH4), a cofactor required for the synthesis of dopamine, serotonin, and other neurotransmitters. QDPR deficiency leads to hyperphenylalaninemia and is associated with atypical PKU, making it relevant to metabolic disease, neuroscience, and enzyme biology research.

References

1. Product information for QDPR Mouse mAb (Ab350667), Aladdin Scientific. Reference code: absea#KC-46464.
2. Blau N, et al. Tetrahydrobiopterin deficiencies without hyperphenylalaninemia: diagnosis and genetics of DHPR deficiency. Journal of Inherited Metabolic Disease. (General reference — specific citation details require verification.)
3. Kaufman S. The phenylalanine hydroxylating system. Advances in Enzymology and Related Areas of Molecular Biology. (General textbook-level reference — specific citation details require verification.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.