SNRPB2 Mouse mAb: Detection of U1 snRNP B'' Protein

SNRPB2 Mouse mAb is a highly specific monoclonal antibody raised against the SNRPB2 protein, a core component of the U1 small nuclear ribonucleoprotein (snRNP) complex essential for pre-mRNA splicing.

Product SKU

Ab378264

Host Species

Mouse

Antibody Format

Monoclonal antibody (mAb)

Concentration

1.00 mg/ml

Source Reference

Abcam40872

Target Protein

SNRPB2 (U1 snRNP B'' protein)

Background: SNRPB2 and the Spliceosome

SNRPB2 (Small Nuclear Ribonucleoprotein Polypeptide B'') is a protein component of the U1 and U2 small nuclear ribonucleoprotein (snRNP) particles, which are integral subunits of the spliceosome. The spliceosome is the large RNA–protein complex responsible for catalyzing the removal of introns from pre-mRNA transcripts, a process called pre-mRNA splicing, which is fundamental to eukaryotic gene expression.

SNRPB2 belongs to the Sm protein family, a group of conserved proteins that form a heptameric ring structure around snRNA molecules. This ring assembly is critical for snRNP biogenesis and stability. Aberrant expression or dysfunction of SNRPB2 and related Sm proteins has been linked to autoimmune conditions and may play roles in oncological contexts, making it a relevant target in both basic and translational research.

Product Specifications

The SNRPB2 Mouse mAb (SKU: Ab378264) is supplied at a concentration of 1.00 mg/ml. This product is sourced with reference to Abcam40872, with batch number F001. Researchers should verify current lot-specific quality control data, including antigen specificity, cross-reactivity profiles, and recommended working dilutions, in the accompanying datasheet.

As a mouse monoclonal antibody, this reagent offers the high batch-to-batch consistency and epitope specificity expected of mAb-based detection tools. The monoclonal format is particularly advantageous in experimental contexts requiring reproducibility across multiple laboratory sites or longitudinal studies.

Recommended Applications and Usage Guidelines

Mouse monoclonal antibodies targeting nuclear ribonucleoproteins such as SNRPB2 are commonly employed in applications including Western blotting (WB), immunofluorescence (IF), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). The specific validated applications for this lot should be confirmed in the product datasheet prior to experimental use.

When designing experiments with SNRPB2 Mouse mAb, researchers should consider the following best practices:

• Sample preparation: For nuclear protein targets, ensure adequate cell lysis and nuclear extraction to maximize antigen availability.
• Blocking conditions: Use appropriate blocking agents (e.g., 5% non-fat dry milk or BSA in TBST) to minimize non-specific background signal.
• Secondary antibody selection: Select an anti-mouse IgG secondary antibody conjugated to the appropriate reporter (HRP, fluorophore) compatible with your detection platform.
• Storage: Store according to datasheet instructions, typically at 4°C for short-term use or −20°C for long-term storage, avoiding repeated freeze–thaw cycles.

Quality and Traceability

The SNRPB2 Mouse mAb (Ab378264) supplied by Aladdin Scientific is traceable to its originating reference (Abcam40872), ensuring a documented chain of custody from manufacture to end use. The recorded batch number (F001) and defined concentration (1.00 mg/ml) support reproducibility documentation required under GLP and institutional quality frameworks.

Researchers performing regulatory or publication-critical experiments are encouraged to retain lot-specific datasheet copies alongside experimental records. Contact Aladdin Scientific technical support for any queries regarding lot-to-lot consistency, certificate of analysis (CoA), or safety data sheets (SDS).

Frequently asked questions

What is the concentration of SNRPB2 Mouse mAb (Ab378264)?

The SNRPB2 Mouse mAb is supplied at a concentration of 1.00 mg/ml, as recorded for batch number F001. Always verify the concentration on the certificate of analysis supplied with your specific lot.

What species is the host for SNRPB2 Mouse mAb?

This antibody is a mouse monoclonal antibody (mAb), meaning it was produced using mouse hybridoma technology. When selecting secondary antibodies, use an anti-mouse IgG secondary appropriate for your detection system.

What is the biological role of SNRPB2, the target of this antibody?

SNRPB2 (Small Nuclear Ribonucleoprotein Polypeptide B'') is a core Sm protein component of U1 and U2 snRNP complexes within the spliceosome. It plays an essential role in pre-mRNA splicing, facilitating the precise removal of introns from nascent transcripts to generate mature mRNA.

How should SNRPB2 Mouse mAb be stored to maintain stability?

As a general guideline for monoclonal antibodies of this type, store at 4°C for short-term use and at −20°C for long-term storage. Avoid repeated freeze–thaw cycles. Always refer to the product-specific datasheet for confirmed storage conditions.

Which applications is SNRPB2 Mouse mAb suitable for?

Mouse monoclonal antibodies against nuclear ribonucleoproteins are commonly used in Western blotting, immunofluorescence, immunoprecipitation, and ELISA. The validated applications for this specific product and lot should be confirmed by consulting the accompanying datasheet or contacting Aladdin Scientific technical support.

References

1. Abcam product reference Abcam40872 – SNRPB2 antibody source documentation. Batch F001.
2. Will CL, Lührmann R. Spliceosome structure and function. Cold Spring Harb Perspect Biol. 2011;3(7):a003707. doi:10.1101/cshperspect.a003707
3. Kastner B, et al. Sm proteins and the biogenesis of small nuclear ribonucleoproteins. In: RNA Processing. Reviews from textbook-level sources on Sm protein ring assembly.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.