Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Cholinesterase detection systems are mainly used to measure the activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and total cholinesterase (ChE). The differences among detection methods are mainly reflected in substrate type, signal generation mode, readout format, ability ...
Thiol and disulfide status are important chemical indicators for evaluating redox balance, protein structural stability, and the degree of stress-induced damage in biological samples. Measuring thiol content alone usually reflects only the level of reactive reduced species under a specific ...
Glutathione (GSH) is one of the most important low-molecular-weight thiols in cells. It participates in reactive oxygen species (ROS) detoxification, maintains protein thiols in a reduced state, drives the glutathione peroxidase/reductase cycle, and—together with oxidized glutathione ...
Ellman’s reagent, 5,5′-dithio-bis-(2-nitrobenzoic acid), also known as DTNB, is a water-soluble compound reacts with free sulfhydryl groups in solutions at slightly alkaline condition to yield a mixed disulfide and highly chromogenic 5-nitro-2- thiobenzoic acid (TNB) with molar extinction ...
Techniques often explored alongside immunological experiments.
