Tema: Protein Biology

Artículos por tema "Protein Biology"

Knowledge Base

Solid-Phase Peptide Synthesis (SPPS) — Efficient Strategies and Innovative Techniques

Solid-phase peptide synthesis (SPPS) is a revolutionary biochemical technique that has become the primary method for synthesizing peptides and proteins since its introduction in the 1960s. Robert Bruce Merrifield first proposed the concept of SPPS in 1963, and this groundbreaking work not only ...

Chemical synthesis Amino acids Protein Biology Acids Solvents Carbohydrates Peptides Peptide synthesis reagents Organic blocks SPPS Peptide synthesis Protection groups

Updated Aug 20248 min read ✓ Reviewed

Protocols

Boc resin lysis protocol

In peptide synthesis, Boc protection groups are widely used to protect the amino terminus of amino acids to prevent unnecessary reactions during synthesis. After the peptide synthesis is complete, the peptide is released from the resin through the cleavage and deprotection steps, and these ...

Building Blocks peptide Protein Biology Solvents Peptides SPPS Peptide synthesis Protection groups Analytical standards

Updated Aug 20248 min read ✓ Reviewed

Technical articles

Method and Precautions of SDS-PAGE Gel Preparation

SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique to separate proteins based on size. By denaturating the protein and giving it a uniform negative charge, the protein migrates to the positive pole under the application of an electric field and is ...

detergent Biological buffer Western blot Protein electrophoresis and Western blotting Protein electrophoresis gel and buffer Oxidant Protein Biology Protein electrophoresis SDS-PAGE

Updated May 20248 min read ✓ Reviewed