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Topic

Biological buffer

Browse protocols, FAQs, guidelines, and technical articles tagged “Biological buffer”, newest first. For research use only.

14 resources · protocols, FAQs, guidelines & articles
Protocol

Protocol of Western Blotting For High Molecular Weights

High molecular weight western blotting is a modification of the standard western blotting technique specifically designed for the detection and analysis of large molecular weight proteins.

Updated ·3 min read
Protocol

Subcellular Fractionation Protocols

Subcellular fractionation is a biological experimental technique that allows researchers to separate and purify different components within cells, such as the nucleus, mitochondria, endoplasmic reticulum, etc., and then further study their structure and function. Centrifugation is one of the ...

Updated ·3 min read
Article

Ethidium Bromide: Beautiful and Dangerous

Ethidium bromide (EtBr) is a classical fluorescent dye widely used in molecular biology research. Its chemical structure consists of a tri-benzimidazole, which can intercalate between the base pairs of DNA or RNA, significantly enhancing its fluorescence signal. Initially discovered as a ...

Updated ·5 min read
Article

The Key to Gene Delivery to Cells —In Vitro Cell Transfection Techniques

Cell transfection techniques are the cornerstone of interdisciplinary research in molecular and cellular biology. They allow scientists to introduce foreign nucleic acids into cells to explore gene function, expression, and develop gene therapies or produce recombinant proteins. This article ...

Updated ·6 min read
Article

Method and Precautions of SDS-PAGE Gel Preparation

SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique to separate proteins based on size. By denaturating the protein and giving it a uniform negative charge, the protein migrates to the positive pole under the application of an electric field and is ...

Updated ·5 min read
Article

Guidelines For Common Cell Culture Media

Cell culture medium is an indispensable part of cell biology research, which provides essential nutrients and a suitable develope environment for cells. The following is a comprehensive introduction to the detailed components and characteristics of several commonly used cell culture media:

Updated ·16 min read
Article

Biological buffer: the unusual amongst the usual

As a common reagent in the pharmaceutical field, biological buffer plays a crucial role in the process of regulating environmental acidity and alkalescence. With the need of epidemic prevention and control, biological buffer as a laboratory routine supplies in the fight against COVID-19 has also ...

Updated ·2 min read

Related topics

Topics that share resources with this page.

Buffer preparation · 3 shared Biochemistry Reagents · 3 shared Good's buffer · 2 shared Zwitterionic Buffer · 2 shared cell culture · 2 shared HEPES · 2 shared Good's Buffers · 2 shared Western blotting · 1 shared Immunoblotting · 1 shared Protein Electrophoresis · 1 shared High Molecular Weights · 1 shared large molecular proteins · 1 shared WB antibody · 1 shared Inorganic catalyst · 1 shared Salt · 3 shared Solvents · 1 shared Molecular Biology · 2 shared Highly pure salt · 1 shared Microbial medium raw material · 1 shared Bacterial growth medium · 1 shared Competence · 1 shared Plasmid transformation · 1 shared E. coli transformation · 1 shared Genetic engineering · 1 shared Calcium chloride · 1 shared Subcellular Fractionation · 1 shared Fractionation Separation · 1 shared Centrifugation · 1 shared Differential Centrifugation · 1 shared Cell and Tissue lysates · 1 shared Fluorescent dyes · 2 shared Fluorescent probes · 1 shared EB · 1 shared Ethidium bromide · 1 shared Chromatography and spectroscopy reagent · 1 shared Nucleic acid dye · 1 shared Electrophoresis reagent · 1 shared Antibiotics · 1 shared Lipids · 1 shared Cell biology · 1 shared Cell transfection · 1 shared In vitro transfection · 1 shared detergent · 1 shared Western blot · 1 shared Protein electrophoresis and Western blotting · 1 shared Protein electrophoresis gel and buffer · 1 shared Oxidant · 1 shared Protein Biology · 1 shared Protein electrophoresis SDS-PAGE · 1 shared Cell medium raw materials · 1 shared Cell culture additives · 1 shared Amino acids · 1 shared Vitamins · 1 shared Growth factors · 1 shared Glucose · 1 shared Nucleosides & nucleotides · 1 shared Carbs · 1 shared Cell culture and transfection · 1 shared Antibody · 2 shared Native ChIP · 1 shared Protein Electrophoresis & Western Blotting · 1 shared Chromosome · 1 shared Immunochemical staining · 1 shared Indirect antibody labeling · 1 shared
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