During cell lysis, tissue homogenization, and protein extraction, endogenous proteases and protein phosphatases are rapidly activated and brought into full contact with target proteins. This leads to protein degradation and the rapid loss of phosphorylation and other post-translational ...
Topic
Protein extraction
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
4 resources · protocols, FAQs & articles
Protein blotting (WB) is widely used to analyze the expression of specific proteins in cell or tissue extracts. Western blot experiments are time-consuming, and their success has a significant impact on research progress.
In the process of nuclear protein extraction, the use of surfactants can significantly interfere with protein labeling efficiency. This is because surfactants can alter the conformation of proteins and may interact with the target protein, affecting its biological activity and the accuracy of ...
This method provides a recipe of lysate and buffer preparation for lysing cells or tissues for western blot analysis.
Related topics
Techniques often explored alongside immunological experiments.