Artículos por tema "Rt-qPCR"

1. Application of Nucleases in Nucleic Acid Sample Processing, Contamination Control, and Molecular Experiment Quality Control
   posted this at 26 may 2026

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   Nucleases are enzymes that catalyze the hydrolysis of phosphodiester bonds in DNA or RNA. They play fundamental roles in nucleic acid extraction, sample purification, contamination control, and molecular experiment quality control.
2. Real-time qPCR Common Probe Chemistries: A Review of Types, Operating Mechanisms, Design Parameters, and Application Strategies
   posted this at 10 feb 2026

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   In real-time quantitative PCR (qPCR) and reverse-transcription real-time quantitative PCR (RT-qPCR), probe chemistries convert target amplification into quantifiable signals through “sequence-specific hybridization–fluorescence switching.” Compared with intercalating dye methods, ...
3. How to efficiently and accurately add samples for rt-qPCR?
   posted this at 9 jun 2025

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   Rt-qPCR (real-time fluorescence quantitative PCR) is a basic experiment in molecular biology, which mainly includes three steps: RNA extraction, reverse transcription to cDNA, and real-time fluorescence quantitative PCR.