Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Nucleases are enzymes that catalyze the hydrolysis of phosphodiester bonds in DNA or RNA. They play fundamental roles in nucleic acid extraction, sample purification, contamination control, and molecular experiment quality control.
In biopharmaceutical processes and high-sensitivity molecular testing systems, the forms in which nucleic acids and nucleases exist and the ways they are controlled directly determine manufacturability, product safety, and analytical credibility.
The DNA of apoptotic cells is cut into fragments of about 200 base pairs under the action of caspase-activated nuclease (CAD), forming DNA ladders. By detecting DNA fragmentation during apoptosis, the degree of apoptosis can be evaluated.
ChIP is a powerful tool that uses isolated chromatin and antibodies against the target antigen to determine whether the target binds to a specific DNA sequence or to map its distribution across the genome (ChIP-seq).
Targets and Release Using Nuclease (CUT&RUN) experimental technology is an emerging chromatin analysis method for studying protein-DNA interactions. It uses antibodies to specifically label target proteins, and then uses nucleases bound to antibodies (such as pAG-MNase) to cut DNA fragments that ...
It is a long-term goal for biomedical researchers to develop efficient and reliable methods to make accurate and targeted changes in the genome of living cells. In recent years, a new tool based on bacterial CRISPR-associated protein-9 nuclease (Cas9) from Streptococcus pyogenes has aroused ...
Techniques often explored alongside immunological experiments.
