Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The core of carbon-nitrogen metabolic crosstalk lies in the dynamic conversion between carbon skeletons and nitrogen flow. Glutamate and α-ketoglutarate form key hubs connecting the TCA cycle, transamination reactions, glutamine metabolism, and amino acid biosynthesis. Their changes reflect not ...
TCA cycle metabolite detection is used to evaluate mitochondrial oxidative metabolism, substrate entry, anaplerotic reactions, redox status, and metabolic reprogramming. Common targets include citrate, α-ketoglutarate, succinate, fumarate, malate, and oxaloacetate. Method selection should be ...
Glucose metabolism is a core network for cellular energy conversion and carbon allocation, spanning glycolysis, gluconeogenesis, glycogen synthesis and breakdown, the pentose phosphate pathway, and the tricarboxylic acid (TCA) cycle.
In neoplastic cells, the de novo synthesis of nucleotides is responsible for maintaining sufficient nucleotide libraries to support DNA replication and RNA production. The metabolic pathways that support nucleotide production are dependent on metabolic intermediates provided by glycolysis and ...
Techniques often explored alongside immunological experiments.
