Antibiotic selection in molecular cloning is not merely a preliminary judgment based on the presence or absence of colonies. Rather, it is a fundamental control point for plasmid maintenance stability, insert integrity, dual-plasmid coexistence, and the reliability of downstream expression. ...
The extensive application of antibiotics in scientific research is evident across various fields. Whether used as selection markers in genetic engineering or to prevent contamination in cell culture, antibiotics have demonstrated their unique value in scientific research.
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