Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Antibiotics are bioactive molecules produced by microorganisms or obtained via semi-synthetic modification that can inhibit or kill bacteria within defined concentration ranges. In research workflows, their core value lies in providing quantifiable, controllable selection pressure and ...
For bacteria to grow, divide, repair damage, and express virulence factors, they must continuously make new proteins. The key machine that “translates” mRNA into protein is the bacterial ribosome. Once translation is stalled, bacteria struggle to keep proliferating and adapting, and ...
Animal tumor models are one of the indispensable technical platforms in modern oncology research, widely used in the fields of tumor occurrence mechanism research, new drug screening, pharmacological and toxicological evaluation, immunotherapy validation, and translational medicine exploration.
The extensive application of antibiotics in scientific research is evident across various fields. Whether used as selection markers in genetic engineering or to prevent contamination in cell culture, antibiotics have demonstrated their unique value in scientific research.
Plasmid transformation is a commonly used technique in molecular biology that introduces foreign plasmid DNA into host cells such as E. coli, enabling gene cloning, gene expression, and other genetic manipulations. Competent E. coli cells are made more permeable to plasmid DNA through physical ...
With the increasing global issue of bacterial resistance and the frequent emergence of new infectious diseases, ribosomally synthesized antimicrobial peptides (RAMPs) have become a promising focus in antibiotic research. Antimicrobial peptides can be categorized into non-ribosomal peptides and ...
Cell transfection techniques are the cornerstone of interdisciplinary research in molecular and cellular biology. They allow scientists to introduce foreign nucleic acids into cells to explore gene function, expression, and develop gene therapies or produce recombinant proteins. This article ...
During protein synthesis in bacteria, antibiotics interfere with ribosome function through specific mechanisms, thereby blocking this crucial step in life activity.
In order to facilitate the experiments of scientific researchers, we have sorted out the buffer formulations commonly used in laboratory basic research in molecular biology, protein biology, microbiology and other aspects for researchers to facilitate access and use.
Techniques often explored alongside immunological experiments.
