Storage buffer: GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6
Product Description:
1. This product is composed of eleven linear double-stranded DNA fragments with a size range of 100bp to 1500bp, specifically 100bp, 200bp, 300bp, 400bp, 500bp, 600bp, 700bp, 800bp, 900bp, 1000bp, and 1500bp. The 500bp band serves as an intensified indicator band, with a concentration 2.5 times higher than that of the other bands, facilitating observation after electrophoresis.
2. In 5μl of this product, the content of the regular bands is approximately 30ng, while the content of the intensified band is about 75ng.
3. This product is already preserved in a 1x Loading Buffer and can be directly used for electrophoresis, offering convenience in use.
4. Both this product and the accompanying 5x Loading Buffer contain the Gelred nucleic acid stain. When used together, after electrophoresis, the bands can be directly observed under ultraviolet light without the need for subsequent staining procedures.
5. This product is not suitable for polyacrylamide gel electrophoresis.
6. Recommended Electrophoresis Buffer and Agarose Gel Concentration:
1x TAE electrophoresis buffer
Agarose concentration: 1.5% to 2.0%.
7. Using this product system, there is no need to add any nucleic acid dye in agarose gel.
Usage Instructions:
1. Prepare an agarose gel of the appropriate concentration without any nucleic acid stains.
2. The concentration of the agarose gel has a significant impact on DNA electrophoresis. The recommended agarose gel concentration for this product is 1.5% to 2.0%.
3. It is suggested to use 1x TAE buffer for electrophoresis, with a voltage not exceeding 10v/cm.
4. For common 3.5mm sample wells, the recommended volume of DNA marker is 3 to 5μl. For wider gel wells, the sample volume should be appropriately increased.
5. Mix the samples to be tested with the accompanying 5x Loading Buffer at a ratio of approximately 4:1, and then load into the gel sample wells.
6. Run the electrophoresis to an appropriate distance:
Since Gelred binds firmly to DNA, it is possible to fully utilize the length of the gel and run a longer distance, as long as the smallest fragment does not run out of the gel, which is beneficial for the separation of small fragments. Generally, the bromophenol blue indicator band should be no less than 1cm away from the edge of the gel.
7. After electrophoresis, observe the electrophoresis bands under a UV lamp.
8. The 5x Loading Buffer included in the product is used for mixing with the samples to be tested before loading, and it contains both bromophenol blue and xylene cyan FF as dual indicators.
9. If there are a large number of samples that can be directly loaded for electrophoresis testing, it is recommended to use the Gelred gel method for detection, without pre-mixing the samples, which can greatly save experimental time.
Product component
| R751624 | Component | 100 T | 500T | Storage | | R751624A | Gelred-prestained DNA Ladder (100-1500bp) | 500 μL
| 5× 500 μL | -20℃. Avoid freeze/thaw cycle. | | R751624B | Gelred-prestained 5xLoading buffer | 500 μL
| 5× 500 μL | -20℃. Avoid freeze/thaw cycle. |
|
