Physical appearance: Liquid
Storage buffer solution: GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6
Product Description:
1. This product is composed of 9 linear double stranded DNA fragments, ranging in size from 100bp to 10000bp, with sizes of 250bp and 500bp respectively 750bp, 1000bp, 1500bp, 2000bp, 3000bp, 5000bp, 10000bp. 1500bp indicates a bright band, with a concentration 2.5 times that of other bands, making it easier to observe after electrophoresis.
2. This product has been saved in a 1xLoading Buffer and can be directly used for electrophoresis, making it easy to use.
3. This product and its accompanying 5xLoading buffer already contain Gelred nucleic acid dye, which can be used in conjunction. After electrophoresis, it can be directly observed under UV without the need for subsequent staining treatment.
4. This product is not suitable for polyacrylamide gel electrophoresis.
5. Recommended concentration of electrophoresis buffer and agarose gel:
1xTAE electrophoresis buffer
Agarose concentration: 1.5%~2.0%,
6. Using this product system, no nucleic acid dye is needed in agarose gel.
instructions:
1. Prepare agarose gel with appropriate concentration and without any nucleic acid dye.
2. The concentration of gel has a great influence on DNA electrophoresis. The recommended concentration of agarose gel for this product is 1.5%~2.0%.
3. It is recommended to use 1xTAE buffer with an electrophoresis voltage not exceeding 10v/cm.
4. The commonly used 3.5mm sample well requires a recommended amount of 3-5 μ l for DNA markers, and the sample size should be appropriately increased for wide gel wells.
5. Mix the sample to be tested with the matching 5xLoading buffer in a ratio of about 4:1, and then add it to the gel sampling hole.
6. Electrophoretic to appropriate distance:
Because Gelred is firmly bound to DNA, it can make full use of the length of gel, and the electrophoresis distance is longer, as long as the smallest segment does not run out of the gel, so as to facilitate the electrophoresis separation of small segments. Generally, the distance between the bromophenol blue indicator band and the edge of gel shall not be less than 1cm.
7. After electrophoresis, observe the electrophoresis bands under ultraviolet light.
8. The 5x loading buffer included in the product is used for sampling after mixing with the sample to be tested, and contains dual indicators of bromophenol blue and xylene blue.
9. If there are a large number of samples that can be directly sampled and require electrophoresis detection, it is recommended to use Gelred gel method for detection without pre mixing samples, which can save a lot of experimental time.
